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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Keratin-associated protein 5-4 (KRTAP5-4) CSB-YP740355HU
CSB-EP740355HU
CSB-BP740355HU
CSB-MP740355HU
CSB-EP740355HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus vulcanus Circadian clock protein kaiA (kaiA) CSB-YP740356TNI
CSB-EP740356TNI
CSB-BP740356TNI
CSB-MP740356TNI
CSB-EP740356TNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Two-component response regulator-like APRR2 (APRR2), partial CSB-YP740357DOA
CSB-EP740357DOA
CSB-BP740357DOA
CSB-MP740357DOA
CSB-EP740357DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP740358LGV
CSB-EP740358LGV
CSB-BP740358LGV
CSB-MP740358LGV
CSB-EP740358LGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Asparagus officinalis Histone H3.2 CSB-YP740359APJ
CSB-EP740359APJ
CSB-BP740359APJ
CSB-MP740359APJ
CSB-EP740359APJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum FMN-dependent NADH-azoreductase 2 (azoR2) CSB-YP740360PIG
CSB-EP740360PIG
CSB-BP740360PIG
CSB-MP740360PIG
CSB-EP740360PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum D-ribose pyranase (rbsD) CSB-YP740361PIG
CSB-EP740361PIG
CSB-BP740361PIG
CSB-MP740361PIG
CSB-EP740361PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Serine hydroxymethyltransferase 2 (glyA2) CSB-YP740362PIG
CSB-EP740362PIG
CSB-BP740362PIG
CSB-MP740362PIG
CSB-EP740362PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP740363PIG
CSB-EP740363PIG
CSB-BP740363PIG
CSB-MP740363PIG
CSB-EP740363PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP740364PIG
CSB-EP740364PIG
CSB-BP740364PIG
CSB-MP740364PIG
CSB-EP740364PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum ATP synthase gamma chain 2 (atpG2) CSB-YP740365PIG
CSB-BP740365PIG
CSB-MP740365PIG
CSB-EP740365PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP740366PIG
CSB-EP740366PIG
CSB-BP740366PIG
CSB-MP740366PIG
CSB-EP740366PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum ATP synthase gamma chain 1 (atpG1) CSB-YP740367PIG
CSB-EP740367PIG
CSB-BP740367PIG
CSB-MP740367PIG
CSB-EP740367PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Protein CyaY (cyaY) CSB-YP740368PIG
CSB-EP740368PIG
CSB-BP740368PIG
CSB-MP740368PIG
CSB-EP740368PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum DNA-binding protein fis (fis) CSB-YP740369PIG
CSB-EP740369PIG
CSB-BP740369PIG
CSB-MP740369PIG
CSB-EP740369PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum tRNA dimethylallyltransferase (miaA) CSB-YP740370PIG
CSB-EP740370PIG
CSB-BP740370PIG
CSB-MP740370PIG
CSB-EP740370PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 30S ribosomal protein S18 (rpsR) CSB-YP740371PIG
CSB-EP740371PIG
CSB-BP740371PIG
CSB-MP740371PIG
CSB-EP740371PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 50S ribosomal protein L13 (rplM) CSB-YP740372PIG
CSB-EP740372PIG
CSB-BP740372PIG
CSB-MP740372PIG
CSB-EP740372PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP740373PIG
CSB-EP740373PIG
CSB-BP740373PIG
CSB-MP740373PIG
CSB-EP740373PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Pantothenate synthetase (panC) CSB-YP740374PIG
CSB-EP740374PIG
CSB-BP740374PIG
CSB-MP740374PIG
CSB-EP740374PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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