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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mesoplasma florum 50S ribosomal protein L36 (rpmJ) CSB-YP739355MEO
CSB-EP739355MEO
CSB-BP739355MEO
CSB-MP739355MEO
CSB-EP739355MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum 50S ribosomal protein L14 (rplN) CSB-YP739356MEO
CSB-EP739356MEO
CSB-BP739356MEO
CSB-MP739356MEO
CSB-EP739356MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum 50S ribosomal protein L2 (rplB) CSB-YP739357MEO
CSB-EP739357MEO
CSB-BP739357MEO
CSB-MP739357MEO
CSB-EP739357MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum ATP synthase subunit beta (atpD) CSB-YP739358MEO
CSB-EP739358MEO
CSB-BP739358MEO
CSB-MP739358MEO
CSB-EP739358MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum 50S ribosomal protein L33 1 (rpmG1) CSB-YP739359MEO
CSB-EP739359MEO
CSB-BP739359MEO
CSB-MP739359MEO
CSB-EP739359MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum Protein translocase subunit SecA (secA), partial CSB-YP739360MEO
CSB-EP739360MEO
CSB-BP739360MEO
CSB-MP739360MEO
CSB-EP739360MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Homeobox protein vex1 (vex1) CSB-YP739361XBF
CSB-EP739361XBF
CSB-BP739361XBF
CSB-MP739361XBF
CSB-EP739361XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Atriplex nummularia S-adenosylmethionine synthase 3 (SAMS3) CSB-YP739362AQU
CSB-EP739362AQU
CSB-BP739362AQU
CSB-MP739362AQU
CSB-EP739362AQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinctada fucata Prismalin-14 CSB-YP739363EVV
CSB-EP739363EVV
CSB-BP739363EVV
CSB-MP739363EVV
CSB-EP739363EVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Leucine-rich repeat-containing protein 16C (RLTPR), partial CSB-YP739364HU
CSB-EP739364HU
CSB-BP739364HU
CSB-MP739364HU
CSB-EP739364HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Putative membrane protein insertion efficiency factor (ACIAD3682) CSB-YP739365AWW
CSB-EP739365AWW
CSB-BP739365AWW
CSB-MP739365AWW
CSB-EP739365AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Chaperone protein DnaJ (dnaJ) CSB-YP739366AWW
CSB-EP739366AWW
CSB-BP739366AWW
CSB-MP739366AWW
CSB-EP739366AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP739367AWW
CSB-EP739367AWW
CSB-BP739367AWW
CSB-MP739367AWW
CSB-EP739367AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP739368AWW
CSB-EP739368AWW
CSB-BP739368AWW
CSB-MP739368AWW
CSB-EP739368AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP739369AWW
CSB-EP739369AWW
CSB-BP739369AWW
CSB-MP739369AWW
CSB-EP739369AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Guanylate kinase (gmk) CSB-YP739370AWW
CSB-EP739370AWW
CSB-BP739370AWW
CSB-MP739370AWW
CSB-EP739370AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 50S ribosomal protein L23 (rplW) CSB-YP739371AWW
CSB-EP739371AWW
CSB-BP739371AWW
CSB-MP739371AWW
CSB-EP739371AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 50S ribosomal protein L30 (rpmD) CSB-YP739372AWW
CSB-EP739372AWW
CSB-BP739372AWW
CSB-MP739372AWW
CSB-EP739372AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 50S ribosomal protein L17 (rplQ) CSB-YP739373AWW
CSB-EP739373AWW
CSB-BP739373AWW
CSB-MP739373AWW
CSB-EP739373AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Protein-export protein SecB (secB) CSB-YP739374AWW
CSB-EP739374AWW
CSB-BP739374AWW
CSB-MP739374AWW
CSB-EP739374AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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