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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bartonella quintana Peptidyl-tRNA hydrolase (pth) CSB-YP739815BSH
CSB-EP739815BSH
CSB-BP739815BSH
CSB-MP739815BSH
CSB-EP739815BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP739816BSH
CSB-EP739816BSH
CSB-BP739816BSH
CSB-MP739816BSH
CSB-EP739816BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP739817BSH
CSB-EP739817BSH
CSB-BP739817BSH
CSB-MP739817BSH
CSB-EP739817BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Recombination protein RecR (recR) CSB-YP739818BSH
CSB-EP739818BSH
CSB-BP739818BSH
CSB-MP739818BSH
CSB-EP739818BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP739819BSH
CSB-EP739819BSH
CSB-BP739819BSH
CSB-MP739819BSH
CSB-EP739819BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Arginine biosynthesis bifunctional protein ArgJ (argJ) CSB-YP739820BSH
CSB-EP739820BSH
CSB-BP739820BSH
CSB-MP739820BSH
CSB-EP739820BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Glycerol-3-phosphate dehydrogenase [NAD (P)+] (gpsA) CSB-YP739821BSH
CSB-EP739821BSH
CSB-BP739821BSH
CSB-MP739821BSH
CSB-EP739821BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana DNA mismatch repair protein MutS (mutS), partial CSB-YP739822BSH
CSB-EP739822BSH
CSB-BP739822BSH
CSB-MP739822BSH
CSB-EP739822BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP739823BSH
CSB-EP739823BSH
CSB-BP739823BSH
CSB-MP739823BSH
CSB-EP739823BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Bifunctional enzyme IspD/IspF (ispDF) CSB-YP739824BSH
CSB-EP739824BSH
CSB-BP739824BSH
CSB-MP739824BSH
CSB-EP739824BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Shikimate dehydrogenase (aroE) CSB-YP739825BSH
CSB-EP739825BSH
CSB-BP739825BSH
CSB-MP739825BSH
CSB-EP739825BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Acetylglutamate kinase (argB) CSB-YP739826BSH
CSB-EP739826BSH
CSB-BP739826BSH
CSB-MP739826BSH
CSB-EP739826BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP739827BSG
CSB-EP739827BSG
CSB-BP739827BSG
CSB-MP739827BSG
CSB-EP739827BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Ribosome maturation factor RimM (rimM) CSB-YP739828BSG
CSB-EP739828BSG
CSB-BP739828BSG
CSB-MP739828BSG
CSB-EP739828BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae ATP synthase subunit alpha (atpA), partial CSB-YP739829BSG
CSB-EP739829BSG
CSB-BP739829BSG
CSB-MP739829BSG
CSB-EP739829BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Translation initiation factor IF-1 (infA) CSB-YP739830BSG
CSB-EP739830BSG
CSB-BP739830BSG
CSB-MP739830BSG
CSB-EP739830BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Type IV secretion system protein virB8 (virB8), partial CSB-YP739831BSG
CSB-EP739831BSG
CSB-BP739831BSG
CSB-MP739831BSG
CSB-EP739831BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae UPF0102 protein BH12350 (BH12350) CSB-YP739832BSG
CSB-EP739832BSG
CSB-BP739832BSG
CSB-MP739832BSG
CSB-EP739832BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP739833BSG
CSB-EP739833BSG
CSB-BP739833BSG
CSB-MP739833BSG
CSB-EP739833BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Ketol-acid reductoisomerase (ilvC) CSB-YP739834BSG
CSB-EP739834BSG
CSB-BP739834BSG
CSB-MP739834BSG
CSB-EP739834BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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