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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S10 (rpsJ) CSB-YP740215BAAE
CSB-EP740215BAAE
CSB-BP740215BAAE
CSB-MP740215BAAE
CSB-EP740215BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L10 (rplJ) CSB-YP740216BAAE
CSB-EP740216BAAE
CSB-BP740216BAAE
CSB-MP740216BAAE
CSB-EP740216BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Bifunctional protein GlmU (glmU) CSB-YP740217BAAE
CSB-EP740217BAAE
CSB-BP740217BAAE
CSB-MP740217BAAE
CSB-EP740217BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0133 protein BT9727_0020 (BT9727_0020) CSB-YP740218BAAE
CSB-EP740218BAAE
CSB-BP740218BAAE
CSB-MP740218BAAE
CSB-EP740218BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0324 membrane protein BA_5405/GBAA_5405/BAS5024 (BA_5405, GBAA_5405, BAS5024), partial CSB-YP740219BQE
CSB-EP740219BQE
CSB-BP740219BQE
CSB-MP740219BQE
CSB-EP740219BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA pseudouridine synthase A 1 (truA1) CSB-YP740220BQE
CSB-EP740220BQE
CSB-BP740220BQE
CSB-MP740220BQE
CSB-EP740220BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cyclin-dependent kinase C-1 (CDKC-1), partial CSB-YP740221OFG
CSB-EP740221OFG
CSB-BP740221OFG
CSB-MP740221OFG
CSB-EP740221OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse E3 ubiquitin-protein ligase HECW2 (Hecw2), partial CSB-YP740222MO
CSB-EP740222MO
CSB-BP740222MO
CSB-MP740222MO
CSB-EP740222MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza C virus Polymerase basic protein 2 (PB2), partial CSB-YP740223IKB
CSB-EP740223IKB
CSB-BP740223IKB
CSB-MP740223IKB
CSB-EP740223IKB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Elongator complex protein 2 (ELP2), partial CSB-YP740224HU
CSB-EP740224HU
CSB-BP740224HU
CSB-MP740224HU
CSB-EP740224HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ret finger protein-like 1 antisense gene protein (RFPL1-AS1) CSB-YP740225HU
CSB-EP740225HU
CSB-BP740225HU
CSB-MP740225HU
CSB-EP740225HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At3g57580 (At3g57580) CSB-YP740226DOA
CSB-EP740226DOA
CSB-BP740226DOA
CSB-MP740226DOA
CSB-EP740226DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Putative inactive serine protease 58 (Prss58) CSB-YP740227RA
CSB-EP740227RA
CSB-BP740227RA
CSB-MP740227RA
CSB-EP740227RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Serine protease inhibitor Kazal-type 2 (Spink2) CSB-YP740228RA
CSB-EP740228RA
CSB-BP740228RA
CSB-MP740228RA
CSB-EP740228RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein Jade-3 (Phf16), partial CSB-YP740229MO
CSB-EP740229MO
CSB-BP740229MO
CSB-MP740229MO
CSB-EP740229MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Olfactory receptor 2T5 (OR2T5), partial CSB-YP740230HU1
CSB-EP740230HU1
CSB-BP740230HU1
CSB-MP740230HU1
CSB-EP740230HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Keratin, type I cytoskeletal 14 (Krt14) CSB-YP740231RA
CSB-EP740231RA
CSB-BP740231RA
CSB-MP740231RA
CSB-EP740231RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Keratin, type I cytoskeletal 39 (Krt39) CSB-YP740232RA
CSB-EP740232RA
CSB-BP740232RA
CSB-MP740232RA
CSB-EP740232RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Keratin, type I cytoskeletal 40 (Krt40) CSB-YP740233MO
CSB-EP740233MO
CSB-BP740233MO
CSB-MP740233MO
CSB-EP740233MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Keratin, type II cytoskeletal 74 (Krt74) CSB-YP740234MO
CSB-EP740234MO
CSB-BP740234MO
CSB-MP740234MO
CSB-EP740234MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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