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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Listeria monocytogenes serotype 4b Putative ATP:guanido phosphotransferase LMOf2365_0243 (LMOf2365_0243) CSB-YP741215LAAD
CSB-EP741215LAAD
CSB-BP741215LAAD
CSB-MP741215LAAD
CSB-EP741215LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b L-lactate dehydrogenase 1 (ldh1) CSB-YP741216LAAD
CSB-EP741216LAAD
CSB-BP741216LAAD
CSB-MP741216LAAD
CSB-EP741216LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP741217LAAD
CSB-EP741217LAAD
CSB-BP741217LAAD
CSB-MP741217LAAD
CSB-EP741217LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 50S ribosomal protein L9 (rplI) CSB-YP741218LAAD
CSB-EP741218LAAD
CSB-BP741218LAAD
CSB-MP741218LAAD
CSB-EP741218LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Putrescine carbamoyltransferase (ptcA) CSB-YP741219LAAD
CSB-EP741219LAAD
CSB-BP741219LAAD
CSB-MP741219LAAD
CSB-EP741219LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Chaperone protein DnaJ (dnaJ) CSB-YP741220DDH
CSB-EP741220DDH
CSB-BP741220DDH
CSB-MP741220DDH
CSB-EP741220DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP741221DDH
CSB-EP741221DDH
CSB-BP741221DDH
CSB-MP741221DDH
CSB-EP741221DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Acetate kinase (ackA) CSB-YP741222DDH
CSB-EP741222DDH
CSB-BP741222DDH
CSB-MP741222DDH
CSB-EP741222DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 50S ribosomal protein L1 (rplA) CSB-YP741223DDH
CSB-EP741223DDH
CSB-BP741223DDH
CSB-MP741223DDH
CSB-EP741223DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Peptide chain release factor 1 (prfA) CSB-YP741224DDH
CSB-EP741224DDH
CSB-BP741224DDH
CSB-MP741224DDH
CSB-EP741224DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris GTP cyclohydrolase folE2 (folE2) CSB-YP741225DDH
CSB-EP741225DDH
CSB-BP741225DDH
CSB-MP741225DDH
CSB-EP741225DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 7-carboxy-7-deazaguanine synthase (queE) CSB-YP741226DDH
CSB-EP741226DDH
CSB-BP741226DDH
CSB-MP741226DDH
CSB-EP741226DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Translation initiation factor IF-3 (infC) CSB-YP741227DDH
CSB-EP741227DDH
CSB-BP741227DDH
CSB-MP741227DDH
CSB-EP741227DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Probable hydrogenase nickel incorporation protein HypA 2 (hypA2) CSB-YP741228DDH
CSB-EP741228DDH
CSB-BP741228DDH
CSB-MP741228DDH
CSB-EP741228DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Chromosomal replication initiator protein DnaA (dnaA) CSB-YP741229DDH
CSB-EP741229DDH
CSB-BP741229DDH
CSB-MP741229DDH
CSB-EP741229DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 10 kDa chaperonin (groS) CSB-YP741230DDH
CSB-EP741230DDH
CSB-BP741230DDH
CSB-MP741230DDH
CSB-EP741230DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Isoleucine--tRNA ligase (ileS), partial CSB-YP741231DDH
CSB-EP741231DDH
CSB-BP741231DDH
CSB-MP741231DDH
CSB-EP741231DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Probable rRNA maturation factor (DVU_1883) CSB-YP741232DDH
CSB-EP741232DDH
CSB-BP741232DDH
CSB-MP741232DDH
CSB-EP741232DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP741233DDH
CSB-EP741233DDH
CSB-BP741233DDH
CSB-MP741233DDH
CSB-EP741233DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Dihydrodipicolinate reductase (dapB) CSB-YP741234DDH
CSB-EP741234DDH
CSB-BP741234DDH
CSB-MP741234DDH
CSB-EP741234DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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