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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Photobacterium profundum Dihydrodipicolinate reductase (dapB) CSB-YP740415PIG
CSB-EP740415PIG
CSB-BP740415PIG
CSB-MP740415PIG
CSB-EP740415PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Lysine--tRNA ligase (lysS), partial CSB-YP740416PIG
CSB-EP740416PIG
CSB-BP740416PIG
CSB-MP740416PIG
CSB-EP740416PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Homoserine kinase (thrB) CSB-YP740417PIG
CSB-EP740417PIG
CSB-BP740417PIG
CSB-MP740417PIG
CSB-EP740417PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP740418PIG
CSB-EP740418PIG
CSB-BP740418PIG
CSB-MP740418PIG
CSB-EP740418PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Isopentenyl-diphosphate Delta-isomerase (idi) CSB-YP740419PIG
CSB-EP740419PIG
CSB-BP740419PIG
CSB-MP740419PIG
CSB-EP740419PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP740420PIG1
CSB-EP740420PIG1
CSB-BP740420PIG1
CSB-MP740420PIG1
CSB-EP740420PIG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Chaperone surA (surA) CSB-YP740421PIG
CSB-EP740421PIG
CSB-BP740421PIG
CSB-MP740421PIG
CSB-EP740421PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP740422PIG
CSB-EP740422PIG
CSB-BP740422PIG
CSB-MP740422PIG
CSB-EP740422PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 30S ribosomal protein S8 (rpsH) CSB-YP740423PIG
CSB-EP740423PIG
CSB-BP740423PIG
CSB-MP740423PIG
CSB-EP740423PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Elongation factor G 1 (fusA1), partial CSB-YP740424PIG
CSB-EP740424PIG
CSB-BP740424PIG
CSB-MP740424PIG
CSB-EP740424PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Protein SlyX homolog (slyX) CSB-YP740425PIG
CSB-EP740425PIG
CSB-BP740425PIG
CSB-MP740425PIG
CSB-EP740425PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 50S ribosomal protein L31 (rpmE) CSB-YP740426PIG
CSB-EP740426PIG
CSB-BP740426PIG
CSB-MP740426PIG
CSB-EP740426PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Glycerol-3-phosphate dehydrogenase [NAD (P)+] (gpsA) CSB-YP740427PIG
CSB-EP740427PIG
CSB-BP740427PIG
CSB-MP740427PIG
CSB-EP740427PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum UPF0758 protein PBPRA0202 (PBPRA0202) CSB-YP740428PIG
CSB-EP740428PIG
CSB-BP740428PIG
CSB-MP740428PIG
CSB-EP740428PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Pimelyl-[acyl-carrier protein] methyl ester esterase (bioH) CSB-YP740429PIG
CSB-EP740429PIG
CSB-BP740429PIG
CSB-MP740429PIG
CSB-EP740429PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP740430PIG
CSB-EP740430PIG
CSB-BP740430PIG
CSB-MP740430PIG
CSB-EP740430PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Ketol-acid reductoisomerase (ilvC) CSB-YP740431PIG
CSB-EP740431PIG
CSB-BP740431PIG
CSB-MP740431PIG
CSB-EP740431PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP740432PIG
CSB-EP740432PIG
CSB-BP740432PIG
CSB-MP740432PIG
CSB-EP740432PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum tRNA modification GTPase MnmE (mnmE) CSB-YP740433PIG
CSB-EP740433PIG
CSB-BP740433PIG
CSB-MP740433PIG
CSB-EP740433PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis CoB--CoM heterodisulfide reductase iron-sulfur subunit A (hdrA), partial CSB-YP740434MSB
CSB-EP740434MSB
CSB-BP740434MSB
CSB-MP740434MSB
CSB-EP740434MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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