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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus UPF0298 protein SAS1056 (SAS1056) CSB-YP739935SKW
CSB-EP739935SKW
CSB-BP739935SKW
CSB-MP739935SKW
CSB-EP739935SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable manganese transport protein MntH (mntH), partial CSB-YP739936SKW
CSB-EP739936SKW
CSB-BP739936SKW
CSB-MP739936SKW
CSB-EP739936SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribonuclease J 1 (SAS1024), partial CSB-YP739937SKW
CSB-EP739937SKW
CSB-BP739937SKW
CSB-MP739937SKW
CSB-EP739937SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphocarrier protein HPr (ptsH) CSB-YP739938SKW
CSB-EP739938SKW
CSB-BP739938SKW
CSB-MP739938SKW
CSB-EP739938SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP739939SKW
CSB-EP739939SKW
CSB-BP739939SKW
CSB-MP739939SKW
CSB-EP739939SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable quinol oxidase subunit 2 (qoxA), partial CSB-YP739940SKW1
CSB-EP739940SKW1
CSB-BP739940SKW1
CSB-MP739940SKW1
CSB-EP739940SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable quinol oxidase subunit 4 (qoxD), partial CSB-YP739941SKW1
CSB-EP739941SKW1
CSB-BP739941SKW1
CSB-MP739941SKW1
CSB-EP739941SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Staphostatin B (sspC) CSB-YP739942SKW
CSB-EP739942SKW
CSB-BP739942SKW
CSB-MP739942SKW
CSB-EP739942SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peptide chain release factor 3 (prfC), partial CSB-YP739943SKW
CSB-EP739943SKW
CSB-BP739943SKW
CSB-MP739943SKW
CSB-EP739943SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Regulatory protein spx (spxA) CSB-YP739944SKW
CSB-EP739944SKW
CSB-BP739944SKW
CSB-MP739944SKW
CSB-EP739944SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Coenzyme A disulfide reductase (cdr) CSB-YP739945SKW
CSB-EP739945SKW
CSB-BP739945SKW
CSB-MP739945SKW
CSB-EP739945SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glucose-6-phosphate isomerase (pgi) CSB-YP739946SKW
CSB-EP739946SKW
CSB-BP739946SKW
CSB-MP739946SKW
CSB-EP739946SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ornithine aminotransferase 2 (rocD2) CSB-YP739947SKW
CSB-EP739947SKW
CSB-BP739947SKW
CSB-MP739947SKW
CSB-EP739947SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Na (+)/H (+) antiporter subunit G1 (mnhG1), partial CSB-YP739948SKW1
CSB-EP739948SKW1
CSB-BP739948SKW1
CSB-MP739948SKW1
CSB-EP739948SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus D-alanine--poly (phosphoribitol) ligase subunit 1 (dltA) CSB-YP739949SKW
CSB-EP739949SKW
CSB-BP739949SKW
CSB-MP739949SKW
CSB-EP739949SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Thermonuclease (nuc) CSB-YP739950SKW
CSB-EP739950SKW
CSB-BP739950SKW
CSB-MP739950SKW
CSB-EP739950SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Enolase (eno) CSB-YP739951SKW
CSB-EP739951SKW
CSB-BP739951SKW
CSB-MP739951SKW
CSB-EP739951SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Thioredoxin reductase (trxB) CSB-YP739952SKW
CSB-EP739952SKW
CSB-BP739952SKW
CSB-MP739952SKW
CSB-EP739952SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HPr kinase/phosphorylase (hprK) CSB-YP739953SKW
CSB-EP739953SKW
CSB-BP739953SKW
CSB-MP739953SKW
CSB-EP739953SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized membrane protein SAS0711 (SAS0711), partial CSB-YP739954SKW1
CSB-EP739954SKW1
CSB-BP739954SKW1
CSB-MP739954SKW1
CSB-EP739954SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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