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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Frog virus 3 Uncharacterized protein 042L (FV3-042L) CSB-YP740135FDG
CSB-EP740135FDG
CSB-BP740135FDG
CSB-MP740135FDG
CSB-EP740135FDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frog virus 3 uncharacterized protein 037R (FV3-037R) CSB-YP740136FDG
CSB-EP740136FDG
CSB-BP740136FDG
CSB-MP740136FDG
CSB-EP740136FDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frog virus 3 Uncharacterized protein 031R (FV3-031R) CSB-YP740137FDG
CSB-EP740137FDG
CSB-BP740137FDG
CSB-MP740137FDG
CSB-EP740137FDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frog virus 3 Uncharacterized protein 017L (FV3-017L), partial CSB-YP740138FDG
CSB-EP740138FDG
CSB-BP740138FDG
CSB-MP740138FDG
CSB-EP740138FDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frog virus 3 Uncharacterized protein 011R (FV3-011R), partial CSB-YP740139FDG
CSB-EP740139FDG
CSB-BP740139FDG
CSB-MP740139FDG
CSB-EP740139FDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frog virus 3 Uncharacterized protein 005R (FV3-005R) CSB-YP740140FDG
CSB-EP740140FDG
CSB-BP740140FDG
CSB-MP740140FDG
CSB-EP740140FDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon 50S ribosomal protein L13 (rplM) CSB-YP740141FCY
CSB-EP740141FCY
CSB-BP740141FCY
CSB-MP740141FCY
CSB-EP740141FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca mulatta Bombesin receptor subtype-3 (BRS3), partial CSB-YP740142MOW1
CSB-EP740142MOW1
CSB-BP740142MOW1
CSB-MP740142MOW1
CSB-EP740142MOW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Potassium transporter 20 (HAK20), partial CSB-YP740143OFG
CSB-EP740143OFG
CSB-BP740143OFG
CSB-MP740143OFG
CSB-EP740143OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Magnesium/proton exchanger 2 (MHX2), partial CSB-YP740144OFG
CSB-EP740144OFG
CSB-BP740144OFG
CSB-MP740144OFG
CSB-EP740144OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica ATP-dependent zinc metalloprotease FTSH 7, chloroplastic (FTSH7), partial CSB-YP740145OFG
CSB-EP740145OFG
CSB-BP740145OFG
CSB-MP740145OFG
CSB-EP740145OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Outer envelope protein 80, chloroplastic (OEP80), partial CSB-YP740146OFG
CSB-EP740146OFG
CSB-BP740146OFG
CSB-MP740146OFG
CSB-EP740146OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Coatomer subunit beta'-2 (Os02g0209100, LOC_Os02g11840), partial CSB-YP740147OFG
CSB-EP740147OFG
CSB-BP740147OFG
CSB-MP740147OFG
CSB-EP740147OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II D2 protein (psbD), partial CSB-YP740148SFAS1
CSB-EP740148SFAS1
CSB-BP740148SFAS1
CSB-MP740148SFAS1
CSB-EP740148SFAS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equisetum fluviatile 30S ribosomal protein S4, chloroplastic (rps4) CSB-YP740149ECAL
CSB-EP740149ECAL
CSB-BP740149ECAL
CSB-MP740149ECAL
CSB-EP740149ECAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Astacin-like metalloendopeptidase (Astl) CSB-YP740150MO
CSB-EP740150MO
CSB-BP740150MO
CSB-MP740150MO
CSB-EP740150MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP740151BAAE
CSB-EP740151BAAE
CSB-BP740151BAAE
CSB-MP740151BAAE
CSB-EP740151BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Thymidine kinase (tdk) CSB-YP740152BAAE
CSB-EP740152BAAE
CSB-BP740152BAAE
CSB-MP740152BAAE
CSB-EP740152BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian ATP synthase epsilon chain (atpC) CSB-YP740153BAAE
CSB-EP740153BAAE
CSB-BP740153BAAE
CSB-MP740153BAAE
CSB-EP740153BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Putative sporulation transcription regulator WhiA (whiA) CSB-YP740154BAAE
CSB-EP740154BAAE
CSB-BP740154BAAE
CSB-MP740154BAAE
CSB-EP740154BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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