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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Protein translocase subunit SecY (secY), partial CSB-YP739995SKV
CSB-EP739995SKV
CSB-BP739995SKV
CSB-MP739995SKV
CSB-EP739995SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L13 (rplM) CSB-YP739996SKV
CSB-EP739996SKV
CSB-BP739996SKV
CSB-MP739996SKV
CSB-EP739996SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tagatose-6-phosphate kinase (lacC) CSB-YP739997SKV
CSB-EP739997SKV
CSB-BP739997SKV
CSB-MP739997SKV
CSB-EP739997SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Multidrug resistance efflux pump sepA (sepA), partial CSB-YP739998SKV1
CSB-EP739998SKV1
CSB-BP739998SKV1
CSB-MP739998SKV1
CSB-EP739998SKV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Deoxyribose-phosphate aldolase 2 (deoC2) CSB-YP739999SKV
CSB-EP739999SKV
CSB-BP739999SKV
CSB-MP739999SKV
CSB-EP739999SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP740000SKV
CSB-EP740000SKV
CSB-BP740000SKV
CSB-MP740000SKV
CSB-EP740000SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0340 protein SAR2202 (SAR2202) CSB-YP740001SKV
CSB-EP740001SKV
CSB-BP740001SKV
CSB-MP740001SKV
CSB-EP740001SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase subunit delta (atpH) CSB-YP740002SKV
CSB-EP740002SKV
CSB-BP740002SKV
CSB-MP740002SKV
CSB-EP740002SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP740003SKV
CSB-EP740003SKV
CSB-BP740003SKV
CSB-MP740003SKV
CSB-EP740003SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Hydroxyethylthiazole kinase (thiM) CSB-YP740004SKV
CSB-EP740004SKV
CSB-BP740004SKV
CSB-MP740004SKV
CSB-EP740004SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP740005SKV
CSB-BP740005SKV
CSB-MP740005SKV
CSB-EP740005SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP740006SKV
CSB-EP740006SKV
CSB-BP740006SKV
CSB-MP740006SKV
CSB-EP740006SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Accessory gene regulator protein B (agrB), partial CSB-YP740007SKV1
CSB-EP740007SKV1
CSB-BP740007SKV1
CSB-MP740007SKV1
CSB-EP740007SKV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA ligase (ligA), partial CSB-YP740008SKV
CSB-EP740008SKV
CSB-BP740008SKV
CSB-MP740008SKV
CSB-EP740008SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Diacylglycerol kinase (dagK) CSB-YP740009SKV
CSB-EP740009SKV
CSB-BP740009SKV
CSB-MP740009SKV
CSB-EP740009SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Response regulator protein vraR (vraR) CSB-YP740010SKV
CSB-EP740010SKV
CSB-BP740010SKV
CSB-MP740010SKV
CSB-EP740010SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Monofunctional glycosyltransferase (mgt), partial CSB-YP740011SKV1
CSB-EP740011SKV1
CSB-BP740011SKV1
CSB-MP740011SKV1
CSB-EP740011SKV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Signal transduction protein TRAP (traP) CSB-YP740012SKV
CSB-EP740012SKV
CSB-BP740012SKV
CSB-MP740012SKV
CSB-EP740012SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus S-adenosylmethionine synthase (metK) CSB-YP740013SKV
CSB-EP740013SKV
CSB-BP740013SKV
CSB-MP740013SKV
CSB-EP740013SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Leucine--tRNA ligase (leuS), partial CSB-YP740014SKV
CSB-EP740014SKV
CSB-BP740014SKV
CSB-MP740014SKV
CSB-EP740014SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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