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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycoplasma mycoides subsp. mycoides SC Triosephosphate isomerase (tpiA) CSB-YP740535MLS
CSB-EP740535MLS
CSB-BP740535MLS
CSB-MP740535MLS
CSB-EP740535MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 30S ribosomal protein S3 (rpsC) CSB-YP740536MLS
CSB-EP740536MLS
CSB-BP740536MLS
CSB-MP740536MLS
CSB-EP740536MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L5 (rplE) CSB-YP740537MLS
CSB-EP740537MLS
CSB-BP740537MLS
CSB-MP740537MLS
CSB-EP740537MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 30S ribosomal protein S13 (rpsM) CSB-YP740538MLS
CSB-EP740538MLS
CSB-BP740538MLS
CSB-MP740538MLS
CSB-EP740538MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Putrescine carbamoyltransferase (ptcA) CSB-YP740539MLS
CSB-EP740539MLS
CSB-BP740539MLS
CSB-MP740539MLS
CSB-EP740539MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Chaperone protein DnaJ (dnaJ) CSB-YP740540MLS
CSB-EP740540MLS
CSB-BP740540MLS
CSB-MP740540MLS
CSB-EP740540MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L27 (rpmA) CSB-YP740541MLS
CSB-EP740541MLS
CSB-BP740541MLS
CSB-MP740541MLS
CSB-EP740541MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Glucose-6-phosphate isomerase (pgi) CSB-YP740542MLS
CSB-EP740542MLS
CSB-BP740542MLS
CSB-MP740542MLS
CSB-EP740542MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Phosphate acyltransferase (plsX) CSB-YP740543MLS
CSB-EP740543MLS
CSB-BP740543MLS
CSB-MP740543MLS
CSB-EP740543MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L19 (rplS) CSB-YP740544MLS
CSB-EP740544MLS
CSB-BP740544MLS
CSB-MP740544MLS
CSB-EP740544MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO 1 (trmFO1) CSB-YP740545MLS
CSB-EP740545MLS
CSB-BP740545MLS
CSB-MP740545MLS
CSB-EP740545MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 30S ribosomal protein S4 (rpsD) CSB-YP740546MLS
CSB-EP740546MLS
CSB-BP740546MLS
CSB-MP740546MLS
CSB-EP740546MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Acetate kinase (ackA) CSB-YP740547MLS
CSB-EP740547MLS
CSB-BP740547MLS
CSB-MP740547MLS
CSB-EP740547MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Elongation factor G (fusA), partial CSB-YP740548MLS
CSB-EP740548MLS
CSB-BP740548MLS
CSB-MP740548MLS
CSB-EP740548MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP740549MLS1
CSB-EP740549MLS1
CSB-BP740549MLS1
CSB-MP740549MLS1
CSB-EP740549MLS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Phosphonates import ATP-binding protein PhnC (phnC) CSB-YP740550MLS
CSB-EP740550MLS
CSB-BP740550MLS
CSB-MP740550MLS
CSB-EP740550MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Thymidylate kinase (tmk) CSB-YP740551MLS
CSB-EP740551MLS
CSB-BP740551MLS
CSB-MP740551MLS
CSB-EP740551MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Porphobilinogen deaminase (hem-3) CSB-YP740552NHA
CSB-EP740552NHA
CSB-BP740552NHA
CSB-MP740552NHA
CSB-EP740552NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Peptidyl-prolyl cis-trans isomerase-like 3 (cyp-10) CSB-YP740553NHA
CSB-EP740553NHA
CSB-BP740553NHA
CSB-MP740553NHA
CSB-EP740553NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata 60S ribosomal protein L17 (rpl17) CSB-YP740554NGS
CSB-EP740554NGS
CSB-BP740554NGS
CSB-MP740554NGS
CSB-EP740554NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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