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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodopseudomonas palustris Bifunctional protein GlmU (glmU) CSB-YP740575RLU
CSB-EP740575RLU
CSB-BP740575RLU
CSB-MP740575RLU
CSB-EP740575RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Methionine import ATP-binding protein MetN 2 (metN2) CSB-YP740576RLU
CSB-EP740576RLU
CSB-BP740576RLU
CSB-MP740576RLU
CSB-EP740576RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris N (2)-fixation sustaining protein CowN (cowN) CSB-YP740577RLU
CSB-EP740577RLU
CSB-BP740577RLU
CSB-MP740577RLU
CSB-EP740577RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Cysteine--tRNA ligase (cysS) CSB-YP740578RLU
CSB-EP740578RLU
CSB-BP740578RLU
CSB-MP740578RLU
CSB-EP740578RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Error-prone DNA polymerase (dnaE2), partial CSB-YP740579RLU
CSB-EP740579RLU
CSB-BP740579RLU
CSB-MP740579RLU
CSB-EP740579RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP740580RLU
CSB-EP740580RLU
CSB-BP740580RLU
CSB-MP740580RLU
CSB-EP740580RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris tRNA (Ile)-lysidine synthase (tilS) CSB-YP740581RLU
CSB-EP740581RLU
CSB-BP740581RLU
CSB-MP740581RLU
CSB-EP740581RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP740582RLU
CSB-EP740582RLU
CSB-BP740582RLU
CSB-MP740582RLU
CSB-EP740582RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP740583RLU
CSB-EP740583RLU
CSB-BP740583RLU
CSB-MP740583RLU
CSB-EP740583RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris ATP synthase subunit b (atpF), partial CSB-YP740584RLU
CSB-EP740584RLU
CSB-BP740584RLU
CSB-MP740584RLU
CSB-EP740584RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Acetylglutamate kinase (argB) CSB-YP740585RLU
CSB-EP740585RLU
CSB-BP740585RLU
CSB-MP740585RLU
CSB-EP740585RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UPF0133 protein RPA0616 (RPA0616) CSB-YP740586RLU
CSB-EP740586RLU
CSB-BP740586RLU
CSB-MP740586RLU
CSB-EP740586RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP740587RLU
CSB-EP740587RLU
CSB-BP740587RLU
CSB-MP740587RLU
CSB-EP740587RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Glycogen synthase (glgA) CSB-YP740588RLU
CSB-EP740588RLU
CSB-BP740588RLU
CSB-MP740588RLU
CSB-EP740588RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Heat-inducible transcription repressor HrcA (hrcA) CSB-YP740589RLU
CSB-EP740589RLU
CSB-BP740589RLU
CSB-MP740589RLU
CSB-EP740589RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP740590RLU
CSB-EP740590RLU
CSB-BP740590RLU
CSB-MP740590RLU
CSB-EP740590RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Leucine--tRNA ligase (leuS), partial CSB-YP740591RLU
CSB-EP740591RLU
CSB-BP740591RLU
CSB-MP740591RLU
CSB-EP740591RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ribosome maturation factor RimM (rimM) CSB-YP740592RLU
CSB-EP740592RLU
CSB-BP740592RLU
CSB-MP740592RLU
CSB-EP740592RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Glutamate 5-kinase (proB) CSB-YP740593RLU
CSB-EP740593RLU
CSB-BP740593RLU
CSB-MP740593RLU
CSB-EP740593RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA (cpdA) CSB-YP740594RLU
CSB-EP740594RLU
CSB-BP740594RLU
CSB-MP740594RLU
CSB-EP740594RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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