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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Protochlamydia amoebophila 60 kDa chaperonin 1 (groL1), partial CSB-YP740495PAAD
CSB-EP740495PAAD
CSB-BP740495PAAD
CSB-MP740495PAAD
CSB-EP740495PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Eukaryotic translation initiation factor 3 subunit G (tif-35) CSB-YP740496NHA
CSB-EP740496NHA
CSB-BP740496NHA
CSB-MP740496NHA
CSB-EP740496NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Tripartite motif-containing protein 40 (Trim40) CSB-YP740497RA
CSB-EP740497RA
CSB-BP740497RA
CSB-MP740497RA
CSB-EP740497RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Lymphocyte antigen 6 complex locus protein G6f (Ly6g6f), partial CSB-YP740498RA
CSB-EP740498RA
CSB-BP740498RA
CSB-MP740498RA
CSB-EP740498RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein Dom3Z (Dom3z) CSB-YP740499RA
CSB-EP740499RA
CSB-BP740499RA
CSB-MP740499RA
CSB-EP740499RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Epidermal growth factor-like protein 8 (Egfl8) CSB-YP740500RA
CSB-EP740500RA
CSB-BP740500RA
CSB-MP740500RA
CSB-EP740500RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Ribosomal RNA small subunit methyltransferase G 3 (rsmG3) CSB-YP740501BFP
CSB-EP740501BFP
CSB-BP740501BFP
CSB-MP740501BFP
CSB-EP740501BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus ATP synthase subunit alpha (atpA), partial CSB-YP740502BFP
CSB-EP740502BFP
CSB-BP740502BFP
CSB-MP740502BFP
CSB-EP740502BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP740503BFP1
CSB-EP740503BFP1
CSB-BP740503BFP1
CSB-MP740503BFP1
CSB-EP740503BFP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Nucleoside diphosphate kinase (ndk) CSB-YP740504BFP
CSB-EP740504BFP
CSB-BP740504BFP
CSB-MP740504BFP
CSB-EP740504BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Putative 3-methyladenine DNA glycosylase (Bd3793) CSB-YP740505BFP
CSB-EP740505BFP
CSB-BP740505BFP
CSB-MP740505BFP
CSB-EP740505BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Aspartate 1-decarboxylase (panD) CSB-YP740506BFP
CSB-EP740506BFP
CSB-BP740506BFP
CSB-MP740506BFP
CSB-EP740506BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Chorismate synthase (aroC) CSB-YP740507BFP
CSB-EP740507BFP
CSB-BP740507BFP
CSB-MP740507BFP
CSB-EP740507BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Ferrochelatase (hemH) CSB-YP740508BFP
CSB-EP740508BFP
CSB-BP740508BFP
CSB-MP740508BFP
CSB-EP740508BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Thymidine kinase (tdk) CSB-YP740509BFP
CSB-EP740509BFP
CSB-BP740509BFP
CSB-MP740509BFP
CSB-EP740509BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP740510BFP
CSB-EP740510BFP
CSB-BP740510BFP
CSB-MP740510BFP
CSB-EP740510BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus 30S ribosomal protein S7 (rpsG) CSB-YP740511BFP
CSB-EP740511BFP
CSB-BP740511BFP
CSB-MP740511BFP
CSB-EP740511BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus 30S ribosomal protein S19 (rpsS) CSB-YP740512BFP
CSB-EP740512BFP
CSB-BP740512BFP
CSB-MP740512BFP
CSB-EP740512BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP740513BFP
CSB-EP740513BFP
CSB-BP740513BFP
CSB-MP740513BFP
CSB-EP740513BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Putative septation protein spoVG (spoVG) CSB-YP740514BFP
CSB-EP740514BFP
CSB-BP740514BFP
CSB-MP740514BFP
CSB-EP740514BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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