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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ashbya gossypii RNA polymerase II subunit A C-terminal domain phosphatase SSU72 (SSU72) CSB-YP741675DOT
CSB-EP741675DOT
CSB-BP741675DOT
CSB-MP741675DOT
CSB-EP741675DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial import inner membrane translocase subunit TIM22 (TIM22), partial CSB-YP741676DOT1
CSB-EP741676DOT1
CSB-BP741676DOT1
CSB-MP741676DOT1
CSB-EP741676DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mediator of RNA polymerase II transcription subunit 31 (SOH1) CSB-YP741677DOT
CSB-EP741677DOT
CSB-BP741677DOT
CSB-MP741677DOT
CSB-EP741677DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein PNS1 (PNS1), partial CSB-YP741678DOT
CSB-EP741678DOT
CSB-BP741678DOT
CSB-MP741678DOT
CSB-EP741678DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Glutamyl-tRNA (Gln) amidotransferase subunit F, mitochondrial (AAR093C), partial CSB-YP741679DOT
CSB-EP741679DOT
CSB-BP741679DOT
CSB-MP741679DOT
CSB-EP741679DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 54S ribosomal protein L2, mitochondrial (MRPL2) CSB-YP741680DOT
CSB-EP741680DOT
CSB-BP741680DOT
CSB-MP741680DOT
CSB-EP741680DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP synthase subunit 5, mitochondrial (ATP5), partial CSB-YP741681DOT
CSB-EP741681DOT
CSB-BP741681DOT
CSB-MP741681DOT
CSB-EP741681DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Squalene monooxygenase (ERG1), partial CSB-YP741682DOT1
CSB-EP741682DOT1
CSB-BP741682DOT1
CSB-MP741682DOT1
CSB-EP741682DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Alanine racemase (alr) CSB-YP741683LEV
CSB-EP741683LEV
CSB-BP741683LEV
CSB-MP741683LEV
CSB-EP741683LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Cobyrinic acid A,C-diamide synthase (cobB) CSB-YP741684LEV
CSB-EP741684LEV
CSB-BP741684LEV
CSB-MP741684LEV
CSB-EP741684LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Cytochrome c oxidase subunit 3 (COX3), partial CSB-YP741685DOT1
CSB-EP741685DOT1
CSB-BP741685DOT1
CSB-MP741685DOT1
CSB-EP741685DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Potassium transporter 21 (HAK21), partial CSB-YP741686OFG
CSB-EP741686OFG
CSB-BP741686OFG
CSB-MP741686OFG
CSB-EP741686OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Chaperone protein ClpB2, chloroplastic (CLPB2), partial CSB-YP741687OFG
CSB-EP741687OFG
CSB-BP741687OFG
CSB-MP741687OFG
CSB-EP741687OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Potassium channel AKT2 (Os05g0428700, LOC_Os05g35410), partial CSB-YP741688OFG
CSB-EP741688OFG
CSB-BP741688OFG
CSB-MP741688OFG
CSB-EP741688OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Beta-glucosidase 8 (BGLU8), partial CSB-YP741689OFG
CSB-EP741689OFG
CSB-BP741689OFG
CSB-MP741689OFG
CSB-EP741689OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Circularly permutated Ras protein 1 (cpras1), partial CSB-YP741690DKK
CSB-EP741690DKK
CSB-BP741690DKK
CSB-MP741690DKK
CSB-EP741690DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Tubulin-specific chaperone A (tbca) CSB-YP741691DKK
CSB-EP741691DKK
CSB-BP741691DKK
CSB-MP741691DKK
CSB-EP741691DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Diphthine synthase (dph5) CSB-YP741692DKK
CSB-EP741692DKK
CSB-BP741692DKK
CSB-MP741692DKK
CSB-EP741692DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Metabotropic glutamate receptor-like protein G (grlG), partial CSB-YP741693DKK1
CSB-EP741693DKK1
CSB-BP741693DKK1
CSB-MP741693DKK1
CSB-EP741693DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum DNA polymerase epsilon subunit 3 (pole3) CSB-YP741694DKK
CSB-EP741694DKK
CSB-BP741694DKK
CSB-MP741694DKK
CSB-EP741694DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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