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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Haemophilus ducreyi tRNA-dihydrouridine synthase A (dusA) CSB-YP742877HSY
CSB-EP742877HSY
CSB-BP742877HSY
CSB-MP742877HSY
CSB-EP742877HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Regulator of ribonuclease activity B (rraB) CSB-YP742878HSY
CSB-EP742878HSY
CSB-BP742878HSY
CSB-MP742878HSY
CSB-EP742878HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Transaldolase (tal) CSB-YP742879HSY
CSB-EP742879HSY
CSB-BP742879HSY
CSB-MP742879HSY
CSB-EP742879HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Malate dehydrogenase (mdh) CSB-YP742880HSY
CSB-EP742880HSY
CSB-BP742880HSY
CSB-MP742880HSY
CSB-EP742880HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Carbamoyl-phosphate synthase small chain (carA) CSB-YP742881HSY
CSB-EP742881HSY
CSB-BP742881HSY
CSB-MP742881HSY
CSB-EP742881HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP742882HSY
CSB-EP742882HSY
CSB-BP742882HSY
CSB-MP742882HSY
CSB-EP742882HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Tryptophan--tRNA ligase (trpS) CSB-YP742883HSY
CSB-EP742883HSY
CSB-BP742883HSY
CSB-MP742883HSY
CSB-EP742883HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP742884HSY
CSB-EP742884HSY
CSB-BP742884HSY
CSB-MP742884HSY
CSB-EP742884HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Uncharacterized protein YjeA (yjeA) CSB-YP742885HSY
CSB-EP742885HSY
CSB-BP742885HSY
CSB-MP742885HSY
CSB-EP742885HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi ATP synthase gamma chain (atpG) CSB-YP742886HSY
CSB-EP742886HSY
CSB-BP742886HSY
CSB-MP742886HSY
CSB-EP742886HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease H (rnhA) CSB-YP742887BHM
CSB-EP742887BHM
CSB-BP742887BHM
CSB-MP742887BHM
CSB-EP742887BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L17 (rplQ) CSB-YP742888BHM
CSB-EP742888BHM
CSB-BP742888BHM
CSB-MP742888BHM
CSB-EP742888BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S5 (rpsE) CSB-YP742889BHM
CSB-EP742889BHM
CSB-BP742889BHM
CSB-MP742889BHM
CSB-EP742889BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L22 (rplV) CSB-YP742890BHM
CSB-EP742890BHM
CSB-BP742890BHM
CSB-MP742890BHM
CSB-EP742890BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP742891BHM
CSB-EP742891BHM
CSB-BP742891BHM
CSB-MP742891BHM
CSB-EP742891BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Siroheme synthase (cysG) CSB-YP742892BHM
CSB-EP742892BHM
CSB-BP742892BHM
CSB-MP742892BHM
CSB-EP742892BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division protein FtsQ (ftsQ), partial CSB-YP742893BHM
CSB-EP742893BHM
CSB-BP742893BHM
CSB-MP742893BHM
CSB-EP742893BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP742894BHM
CSB-EP742894BHM
CSB-BP742894BHM
CSB-MP742894BHM
CSB-EP742894BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP742895BHM
CSB-EP742895BHM
CSB-BP742895BHM
CSB-MP742895BHM
CSB-EP742895BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB (rlmB) CSB-YP742896BHM
CSB-EP742896BHM
CSB-BP742896BHM
CSB-MP742896BHM
CSB-EP742896BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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