Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ashbya gossypii Chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), partial CSB-YP741655DOT
CSB-EP741655DOT
CSB-BP741655DOT
CSB-MP741655DOT
CSB-EP741655DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ASTRA-associated protein 1 (ASA1) CSB-YP741656DOT
CSB-EP741656DOT
CSB-BP741656DOT
CSB-MP741656DOT
CSB-EP741656DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Oleate activated transcription factor 3 (OAF3), partial CSB-YP741657DOT
CSB-EP741657DOT
CSB-BP741657DOT
CSB-MP741657DOT
CSB-EP741657DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Glutamine synthetase (GLN1) CSB-YP741658DOT
CSB-EP741658DOT
CSB-BP741658DOT
CSB-MP741658DOT
CSB-EP741658DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Serine/threonine-protein phosphatase 2A activator 2 (RRD2) CSB-YP741659DOT
CSB-EP741659DOT
CSB-BP741659DOT
CSB-MP741659DOT
CSB-EP741659DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Altered inheritance of mitochondria protein 41, mitochondrial (AIM41) CSB-YP741660DOT
CSB-EP741660DOT
CSB-BP741660DOT
CSB-MP741660DOT
CSB-EP741660DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Sorting nexin-4 (SNX4) CSB-YP741661DOT
CSB-EP741661DOT
CSB-BP741661DOT
CSB-MP741661DOT
CSB-EP741661DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DNA ligase 4 (LIG4), partial CSB-YP741662DOT
CSB-EP741662DOT
CSB-BP741662DOT
CSB-MP741662DOT
CSB-EP741662DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ERAD-associated E3 ubiquitin-protein ligase HRD1 (HRD1), partial CSB-YP741663DOT1
CSB-EP741663DOT1
CSB-BP741663DOT1
CSB-MP741663DOT1
CSB-EP741663DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDH2), partial CSB-YP741664DOT
CSB-EP741664DOT
CSB-BP741664DOT
CSB-MP741664DOT
CSB-EP741664DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Cargo-transport protein YPP1 (YPP1), partial CSB-YP741665DOT
CSB-EP741665DOT
CSB-BP741665DOT
CSB-MP741665DOT
CSB-EP741665DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein transport protein BOS1 (BOS1), partial CSB-YP741666DOT
CSB-EP741666DOT
CSB-BP741666DOT
CSB-MP741666DOT
CSB-EP741666DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Palmitoyltransferase PFA5 (PFA5), partial CSB-YP741667DOT1
CSB-EP741667DOT1
CSB-BP741667DOT1
CSB-MP741667DOT1
CSB-EP741667DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Serine/threonine-protein kinase BUR1 (BUR1), partial CSB-YP741668DOT
CSB-EP741668DOT
CSB-BP741668DOT
CSB-MP741668DOT
CSB-EP741668DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pro-apoptotic serine protease NMA111 (NMA111), partial CSB-YP741669DOT
CSB-EP741669DOT
CSB-BP741669DOT
CSB-MP741669DOT
CSB-EP741669DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Nucleoside diphosphate kinase (NDK1) CSB-YP741670DOT
CSB-EP741670DOT
CSB-BP741670DOT
CSB-MP741670DOT
CSB-EP741670DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 40S ribosomal protein S25 (RPS25) CSB-YP741671DOT
CSB-EP741671DOT
CSB-BP741671DOT
CSB-MP741671DOT
CSB-EP741671DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii F-actin-capping protein subunit alpha (CAP1) CSB-YP741672DOT
CSB-EP741672DOT
CSB-BP741672DOT
CSB-MP741672DOT
CSB-EP741672DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial genome maintenance protein MGM101 (MGM101) CSB-YP741673DOT
CSB-EP741673DOT
CSB-BP741673DOT
CSB-MP741673DOT
CSB-EP741673DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii COP9 signalosome complex subunit 5 (RRI1) CSB-YP741674DOT
CSB-EP741674DOT
CSB-BP741674DOT
CSB-MP741674DOT
CSB-EP741674DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>