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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chromobacterium violaceum Ribonuclease P protein component (rnpA) CSB-YP742316CKA
CSB-EP742316CKA
CSB-BP742316CKA
CSB-MP742316CKA
CSB-EP742316CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP742317CKA
CSB-EP742317CKA
CSB-BP742317CKA
CSB-MP742317CKA
CSB-EP742317CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP742318CKA
CSB-EP742318CKA
CSB-BP742318CKA
CSB-MP742318CKA
CSB-EP742318CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Protein smg homolog (smg) CSB-YP742319CKA
CSB-EP742319CKA
CSB-BP742319CKA
CSB-MP742319CKA
CSB-EP742319CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP742320CKA
CSB-EP742320CKA
CSB-BP742320CKA
CSB-MP742320CKA
CSB-EP742320CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP742321CKA
CSB-EP742321CKA
CSB-BP742321CKA
CSB-MP742321CKA
CSB-EP742321CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 50S ribosomal protein L2 (rplB) CSB-YP742322CKA
CSB-EP742322CKA
CSB-BP742322CKA
CSB-MP742322CKA
CSB-EP742322CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 50S ribosomal protein L24 (rplX) CSB-YP742323CKA
CSB-EP742323CKA
CSB-BP742323CKA
CSB-MP742323CKA
CSB-EP742323CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 50S ribosomal protein L15 (rplO) CSB-YP742324CKA
CSB-EP742324CKA
CSB-BP742324CKA
CSB-MP742324CKA
CSB-EP742324CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Apolipoprotein N-acyltransferase (lnt), partial CSB-YP742325CKA1
CSB-EP742325CKA1
CSB-BP742325CKA1
CSB-MP742325CKA1
CSB-EP742325CKA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP742326CKA
CSB-EP742326CKA
CSB-BP742326CKA
CSB-MP742326CKA
CSB-EP742326CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Acetylglutamate kinase (argB) CSB-YP742327CKA
CSB-EP742327CKA
CSB-BP742327CKA
CSB-MP742327CKA
CSB-EP742327CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP742328CKA
CSB-EP742328CKA
CSB-BP742328CKA
CSB-MP742328CKA
CSB-EP742328CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP742329CKA
CSB-EP742329CKA
CSB-BP742329CKA
CSB-MP742329CKA
CSB-EP742329CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Putative phosphotransferase CV_3710 (CV_3710) CSB-YP742330CKA
CSB-EP742330CKA
CSB-BP742330CKA
CSB-MP742330CKA
CSB-EP742330CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP742331CKA
CSB-EP742331CKA
CSB-BP742331CKA
CSB-MP742331CKA
CSB-EP742331CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP742332CKA
CSB-EP742332CKA
CSB-BP742332CKA
CSB-MP742332CKA
CSB-EP742332CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP742333CKA
CSB-EP742333CKA
CSB-BP742333CKA
CSB-MP742333CKA
CSB-EP742333CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Nucleoside diphosphate kinase (ndk) CSB-YP742334CKA
CSB-EP742334CKA
CSB-BP742334CKA
CSB-MP742334CKA
CSB-EP742334CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UPF0276 protein CV_3513 (CV_3513) CSB-YP742335CKA
CSB-EP742335CKA
CSB-BP742335CKA
CSB-MP742335CKA
CSB-EP742335CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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