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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus laevis Methionine--tRNA ligase, cytoplasmic (mars), partial CSB-YP744117XBE
CSB-EP744117XBE
CSB-BP744117XBE
CSB-MP744117XBE
CSB-EP744117XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Selenide, water dikinase 1 (sephs1) CSB-YP744118XBE
CSB-EP744118XBE
CSB-BP744118XBE
CSB-MP744118XBE
CSB-EP744118XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse DNA repair and recombination protein RAD54B (Rad54b), partial CSB-YP744119MO
CSB-EP744119MO
CSB-BP744119MO
CSB-MP744119MO
CSB-EP744119MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse DDB1- and CUL4-associated factor 15 (Dcaf15) CSB-YP744120MO
CSB-EP744120MO
CSB-BP744120MO
CSB-MP744120MO
CSB-EP744120MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio WD repeat-containing protein 48 (wdr48), partial CSB-YP744121DIL
CSB-EP744121DIL
CSB-BP744121DIL
CSB-MP744121DIL
CSB-EP744121DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ras GTPase-activating protein 4 (Rasa4), partial CSB-YP744122MO
CSB-EP744122MO
CSB-BP744122MO
CSB-MP744122MO
CSB-EP744122MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Amyloid beta A4 precursor protein-binding family B member 1-interacting protein (apbb1ip), partial CSB-YP744123DIL
CSB-EP744123DIL
CSB-BP744123DIL
CSB-MP744123DIL
CSB-EP744123DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse N-acetylaspartyl-glutamate synthetase A (Rimkla) CSB-YP744124MO
CSB-EP744124MO
CSB-BP744124MO
CSB-MP744124MO
CSB-EP744124MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio RNA-binding protein with serine-rich domain 1 (rnps1) CSB-YP744125DIL
CSB-EP744125DIL
CSB-BP744125DIL
CSB-MP744125DIL
CSB-EP744125DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Solute carrier family 35 member E3 (Slc35e3), partial CSB-YP744126MO
CSB-EP744126MO
CSB-BP744126MO
CSB-MP744126MO
CSB-EP744126MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse MORN repeat-containing protein 4 (Morn4) CSB-YP744127MO
CSB-EP744127MO
CSB-BP744127MO
CSB-MP744127MO
CSB-EP744127MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein C22orf43 (C22orf43) CSB-YP744128HU
CSB-EP744128HU
CSB-BP744128HU
CSB-MP744128HU
CSB-EP744128HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Serotransferrin-B (tf-b), partial CSB-YP744129XBE
CSB-EP744129XBE
CSB-BP744129XBE
CSB-MP744129XBE
CSB-EP744129XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cytosolic sulfotransferase 1 (sult1st1) CSB-YP744130DIL
CSB-EP744130DIL
CSB-BP744130DIL
CSB-MP744130DIL
CSB-EP744130DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Interleukin-20 receptor subunit alpha (Il20ra), partial CSB-YP744131MO
CSB-EP744131MO
CSB-BP744131MO
CSB-MP744131MO
CSB-EP744131MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Sodium/potassium-transporting ATPase subunit beta-1-interacting protein 1 (nkain1), partial CSB-YP744132DIL1
CSB-EP744132DIL1
CSB-BP744132DIL1
CSB-MP744132DIL1
CSB-EP744132DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ras-related protein Rab-35 (Rab35) CSB-YP744133MO
CSB-EP744133MO
CSB-BP744133MO
CSB-MP744133MO
CSB-EP744133MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein SHQ1 homolog (SHQ1) CSB-YP744134HU
CSB-EP744134HU
CSB-BP744134HU
CSB-MP744134HU
CSB-EP744134HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human F-box only protein 38 (FBXO38), partial CSB-YP744135HU
CSB-EP744135HU
CSB-BP744135HU
CSB-MP744135HU
CSB-EP744135HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human F-box only protein 46 (FBXO46) CSB-YP744136HU
CSB-EP744136HU
CSB-BP744136HU
CSB-MP744136HU
CSB-EP744136HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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