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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Photorhabdus luminescens subsp. laumondii Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP742236PIJ
CSB-EP742236PIJ
CSB-BP742236PIJ
CSB-MP742236PIJ
CSB-EP742236PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii tRNA (Ile)-lysidine synthase (tilS) CSB-YP742237PIJ
CSB-EP742237PIJ
CSB-BP742237PIJ
CSB-MP742237PIJ
CSB-EP742237PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Undecaprenyl pyrophosphate synthase (uppS) CSB-YP742238PIJ
CSB-EP742238PIJ
CSB-BP742238PIJ
CSB-MP742238PIJ
CSB-EP742238PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 30S ribosomal protein S2 (rpsB) CSB-YP742239PIJ
CSB-EP742239PIJ
CSB-BP742239PIJ
CSB-MP742239PIJ
CSB-EP742239PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP742240PIJ
CSB-EP742240PIJ
CSB-BP742240PIJ
CSB-MP742240PIJ
CSB-EP742240PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii LPS-assembly protein lptD (lptD), partial CSB-YP742241PIJ
CSB-EP742241PIJ
CSB-BP742241PIJ
CSB-MP742241PIJ
CSB-EP742241PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Lipoprotein signal peptidase (lspA), partial CSB-YP742242PIJ1
CSB-EP742242PIJ1
CSB-BP742242PIJ1
CSB-MP742242PIJ1
CSB-EP742242PIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Homoserine kinase (thrB) CSB-YP742243PIJ
CSB-EP742243PIJ
CSB-BP742243PIJ
CSB-MP742243PIJ
CSB-EP742243PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Endonuclease V (nfi) CSB-YP742244PIJ
CSB-EP742244PIJ
CSB-BP742244PIJ
CSB-MP742244PIJ
CSB-EP742244PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP742245PIJ
CSB-EP742245PIJ
CSB-BP742245PIJ
CSB-MP742245PIJ
CSB-EP742245PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Sulfurtransferase TusD (tusD) CSB-YP742246PIJ
CSB-EP742246PIJ
CSB-BP742246PIJ
CSB-MP742246PIJ
CSB-EP742246PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii DNA ligase B (ligB), partial CSB-YP742247PIJ
CSB-EP742247PIJ
CSB-BP742247PIJ
CSB-MP742247PIJ
CSB-EP742247PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Pimelyl-[acyl-carrier protein] methyl ester esterase (bioH) CSB-YP742248PIJ
CSB-EP742248PIJ
CSB-BP742248PIJ
CSB-MP742248PIJ
CSB-EP742248PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Cyanate hydratase (cynS) CSB-YP742249PIJ
CSB-EP742249PIJ
CSB-BP742249PIJ
CSB-MP742249PIJ
CSB-EP742249PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii D-ribose pyranase (rbsD) CSB-YP742250PIJ
CSB-EP742250PIJ
CSB-BP742250PIJ
CSB-MP742250PIJ
CSB-EP742250PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii ATP synthase subunit alpha (atpA), partial CSB-YP742251PIJ
CSB-EP742251PIJ
CSB-BP742251PIJ
CSB-MP742251PIJ
CSB-EP742251PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii DNA replication and repair protein recF (recF) CSB-YP742252PIJ
CSB-EP742252PIJ
CSB-BP742252PIJ
CSB-MP742252PIJ
CSB-EP742252PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP742253MWI1
CSB-EP742253MWI1
CSB-BP742253MWI1
CSB-MP742253MWI1
CSB-EP742253MWI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Aspartate--ammonia ligase (asnA) CSB-YP742254MWI
CSB-EP742254MWI
CSB-BP742254MWI
CSB-MP742254MWI
CSB-EP742254MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Deoxyribose-phosphate aldolase (deoC) CSB-YP742255MWI
CSB-EP742255MWI
CSB-BP742255MWI
CSB-MP742255MWI
CSB-EP742255MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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