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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO (trmFO) CSB-YP743517BAAE
CSB-EP743517BAAE
CSB-BP743517BAAE
CSB-MP743517BAAE
CSB-EP743517BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ribosome maturation factor RimP (rimP) CSB-YP743518BAAE
CSB-EP743518BAAE
CSB-BP743518BAAE
CSB-MP743518BAAE
CSB-EP743518BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP743519BAAE
CSB-EP743519BAAE
CSB-BP743519BAAE
CSB-MP743519BAAE
CSB-EP743519BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian LexA repressor (lexA) CSB-YP743520BAAE
CSB-EP743520BAAE
CSB-BP743520BAAE
CSB-MP743520BAAE
CSB-EP743520BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Small, acid-soluble spore protein tlp (tlp) CSB-YP743521BAAE
CSB-EP743521BAAE
CSB-BP743521BAAE
CSB-MP743521BAAE
CSB-EP743521BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phosphoserine aminotransferase (serC) CSB-YP743522BAAE
CSB-EP743522BAAE
CSB-BP743522BAAE
CSB-MP743522BAAE
CSB-EP743522BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Probable RNA 2'-phosphotransferase (kptA) CSB-YP743523BAAE
CSB-EP743523BAAE
CSB-BP743523BAAE
CSB-MP743523BAAE
CSB-EP743523BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Aliphatic sulfonates import ATP-binding protein SsuB (ssuB) CSB-YP743524BAAE
CSB-EP743524BAAE
CSB-BP743524BAAE
CSB-MP743524BAAE
CSB-EP743524BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Inosose dehydratase (iolE) CSB-YP743525BAAE
CSB-EP743525BAAE
CSB-BP743525BAAE
CSB-MP743525BAAE
CSB-EP743525BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0346 protein BT9727_2085 (BT9727_2085) CSB-YP743526BAAE
CSB-EP743526BAAE
CSB-BP743526BAAE
CSB-MP743526BAAE
CSB-EP743526BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Homoserine kinase (thrB) CSB-YP743527BAAE
CSB-EP743527BAAE
CSB-BP743527BAAE
CSB-MP743527BAAE
CSB-EP743527BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0291 protein BT9727_1737 (BT9727_1737) CSB-YP743528BAAE
CSB-EP743528BAAE
CSB-BP743528BAAE
CSB-MP743528BAAE
CSB-EP743528BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Cell cycle protein GpsB (gpsB) CSB-YP743529BAAE
CSB-EP743529BAAE
CSB-BP743529BAAE
CSB-MP743529BAAE
CSB-EP743529BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Dihydrodipicolinate reductase (dapB) CSB-YP743530BAAE
CSB-EP743530BAAE
CSB-BP743530BAAE
CSB-MP743530BAAE
CSB-EP743530BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Nucleoside diphosphate kinase (ndk) CSB-YP743531BAAE
CSB-EP743531BAAE
CSB-BP743531BAAE
CSB-MP743531BAAE
CSB-EP743531BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP743532BAAE
CSB-EP743532BAAE
CSB-BP743532BAAE
CSB-MP743532BAAE
CSB-EP743532BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP743533BAAE
CSB-EP743533BAAE
CSB-BP743533BAAE
CSB-MP743533BAAE
CSB-EP743533BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian ATP phosphoribosyltransferase (hisG) CSB-YP743534BAAE
CSB-EP743534BAAE
CSB-BP743534BAAE
CSB-MP743534BAAE
CSB-EP743534BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian D-alanine--poly (phosphoribitol) ligase subunit 1 (dltA), partial CSB-YP743535BAAE
CSB-EP743535BAAE
CSB-BP743535BAAE
CSB-MP743535BAAE
CSB-EP743535BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Spermidine/putrescine import ATP-binding protein PotA (potA) CSB-YP743536BAAE
CSB-EP743536BAAE
CSB-BP743536BAAE
CSB-MP743536BAAE
CSB-EP743536BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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