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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus laevis Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6-A (jmjd6-a) CSB-YP743437XBE
CSB-EP743437XBE
CSB-BP743437XBE
CSB-MP743437XBE
CSB-EP743437XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Cytosolic 10-formyltetrahydrofolate dehydrogenase (aldh1l1), partial CSB-YP743438XBE
CSB-EP743438XBE
CSB-BP743438XBE
CSB-MP743438XBE
CSB-EP743438XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Charged multivesicular body protein 4c (chmp4c) CSB-YP743439XBE
CSB-EP743439XBE
CSB-BP743439XBE
CSB-MP743439XBE
CSB-EP743439XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Disrupted in renal carcinoma protein 2 homolog (dirc2), partial CSB-YP743440XBE
CSB-EP743440XBE
CSB-BP743440XBE
CSB-MP743440XBE
CSB-EP743440XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein FAM54B-A (fam54b-a) CSB-YP743441XBE
CSB-EP743441XBE
CSB-BP743441XBE
CSB-MP743441XBE
CSB-EP743441XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Lariat debranching enzyme A (dbr1-a), partial CSB-YP743442XBE
CSB-EP743442XBE
CSB-BP743442XBE
CSB-MP743442XBE
CSB-EP743442XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Leucine-rich repeat-containing protein 40 (lrrc40), partial CSB-YP743443XBE
CSB-EP743443XBE
CSB-BP743443XBE
CSB-MP743443XBE
CSB-EP743443XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Transmembrane protein 150A (tmem150a), partial CSB-YP743444XBE1
CSB-EP743444XBE1
CSB-BP743444XBE1
CSB-MP743444XBE1
CSB-EP743444XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Serine/threonine-protein kinase Sgk1-A (sgk1-a) CSB-YP743445XBE
CSB-EP743445XBE
CSB-BP743445XBE
CSB-MP743445XBE
CSB-EP743445XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis UPF0533 protein C5orf44 homolog CSB-YP743446XBE
CSB-EP743446XBE
CSB-BP743446XBE
CSB-MP743446XBE
CSB-EP743446XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Mitochondrial import inner membrane translocase subunit Tim13-A (timm13-a) CSB-YP743447XBE
CSB-EP743447XBE
CSB-BP743447XBE
CSB-MP743447XBE
CSB-EP743447XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis RISC-loading complex subunit tarbp2 (tarbp2) CSB-YP743448XBE
CSB-EP743448XBE
CSB-BP743448XBE
CSB-MP743448XBE
CSB-EP743448XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gamma-aminobutyric acid receptor-associated protein-like 1 (gabarapl1) CSB-YP743449XBE
CSB-EP743449XBE
CSB-BP743449XBE
CSB-MP743449XBE
CSB-EP743449XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis LSM domain-containing protein 1-A (lsmd1-a) CSB-YP743450XBE
CSB-EP743450XBE
CSB-BP743450XBE
CSB-MP743450XBE
CSB-EP743450XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Mediator of RNA polymerase II transcription subunit 31-B (med31-b) CSB-YP743451XBE
CSB-EP743451XBE
CSB-BP743451XBE
CSB-MP743451XBE
CSB-EP743451XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis AT-rich interactive domain-containing protein 3A (arid3a), partial CSB-YP743452XBE
CSB-EP743452XBE
CSB-BP743452XBE
CSB-MP743452XBE
CSB-EP743452XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Probable arginine--tRNA ligase, mitochondrial (rars2), partial CSB-YP743453XBE
CSB-EP743453XBE
CSB-BP743453XBE
CSB-MP743453XBE
CSB-EP743453XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Trans-2-enoyl-CoA reductase, mitochondrial (mecr) CSB-YP743454DIL
CSB-EP743454DIL
CSB-BP743454DIL
CSB-MP743454DIL
CSB-EP743454DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Uncharacterized protein C16orf59 homolog CSB-YP743455MO
CSB-EP743455MO
CSB-BP743455MO
CSB-MP743455MO
CSB-EP743455MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Embryonic polyadenylate-binding protein B (epabp-b), partial CSB-YP743456XBE
CSB-EP743456XBE
CSB-BP743456XBE
CSB-MP743456XBE
CSB-EP743456XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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