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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 50S ribosomal protein L5 (rplE) CSB-YP745239SMU
CSB-EP745239SMU
CSB-BP745239SMU
CSB-MP745239SMU
CSB-EP745239SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Lectin-like protein BA14k (BMEII0552), partial CSB-YP745240BMO1
CSB-EP745240BMO1
CSB-BP745240BMO1
CSB-MP745240BMO1
CSB-EP745240BMO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Porin omp2a (omp2a) CSB-YP745241BMO
CSB-EP745241BMO
CSB-BP745241BMO
CSB-MP745241BMO
CSB-EP745241BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Quaternary ammonium compound-resistance protein sugE (sugE), partial CSB-YP745242SXB1
CSB-EP745242SXB1
CSB-BP745242SXB1
CSB-MP745242SXB1
CSB-EP745242SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium L-rhamnose mutarotase (rhaM) CSB-YP745243SXB
CSB-EP745243SXB
CSB-BP745243SXB
CSB-MP745243SXB
CSB-EP745243SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Small heat shock protein ibpA (ibpA) CSB-YP745244SXB
CSB-EP745244SXB
CSB-BP745244SXB
CSB-MP745244SXB
CSB-EP745244SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 50S ribosomal protein L16 (rplP) CSB-YP745245SXB
CSB-EP745245SXB
CSB-BP745245SXB
CSB-MP745245SXB
CSB-EP745245SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Lipoprotein NlpI (nlpI) CSB-YP745246SXB
CSB-EP745246SXB
CSB-BP745246SXB
CSB-MP745246SXB
CSB-EP745246SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Cell division protein ZapA (zapA) CSB-YP745247SXB
CSB-EP745247SXB
CSB-BP745247SXB
CSB-MP745247SXB
CSB-EP745247SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Chaperone protein ClpB (clpB), partial CSB-YP745248SXB
CSB-EP745248SXB
CSB-BP745248SXB
CSB-MP745248SXB
CSB-EP745248SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium GDP-mannose pyrophosphatase nudK (nudK) CSB-YP745249SXB
CSB-EP745249SXB
CSB-BP745249SXB
CSB-MP745249SXB
CSB-EP745249SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 50S ribosomal protein L25 (rplY) CSB-YP745250SXB
CSB-EP745250SXB
CSB-BP745250SXB
CSB-MP745250SXB
CSB-EP745250SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Endo-type membrane-bound lytic murein transglycosylase A (emtA) CSB-YP745251SXB
CSB-EP745251SXB
CSB-BP745251SXB
CSB-MP745251SXB
CSB-EP745251SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Major outer membrane lipoprotein 1 (lpp1) CSB-YP745252SXB
CSB-BP745252SXB
CSB-MP745252SXB
CSB-EP745252SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Acylphosphatase (yccX) CSB-YP745253SXB
CSB-EP745253SXB
CSB-BP745253SXB
CSB-MP745253SXB
CSB-EP745253SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Negative modulator of initiation of replication (seqA) CSB-YP745254SXB
CSB-EP745254SXB
CSB-BP745254SXB
CSB-MP745254SXB
CSB-EP745254SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Anti-adapter protein iraP (iraP) CSB-YP745255SXB
CSB-EP745255SXB
CSB-BP745255SXB
CSB-MP745255SXB
CSB-EP745255SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Chaperone surA (surA) CSB-YP745256SXB
CSB-EP745256SXB
CSB-BP745256SXB
CSB-MP745256SXB
CSB-EP745256SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Chaperone protein ClpB (clpB), partial CSB-YP745257AYS
CSB-EP745257AYS
CSB-BP745257AYS
CSB-MP745257AYS
CSB-EP745257AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens ATP synthase subunit delta (atpH) CSB-YP745258AYS
CSB-EP745258AYS
CSB-BP745258AYS
CSB-MP745258AYS
CSB-EP745258AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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