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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase (MAP_1275) CSB-YP744839MLA
CSB-EP744839MLA
CSB-BP744839MLA
CSB-MP744839MLA
CSB-EP744839MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Vpx (vpx) CSB-YP744840HDAG
CSB-EP744840HDAG
CSB-BP744840HDAG
CSB-MP744840HDAG
CSB-EP744840HDAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protoheme IX farnesyltransferase (ctaB), partial CSB-YP744841MLA
CSB-EP744841MLA
CSB-BP744841MLA
CSB-MP744841MLA
CSB-EP744841MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionyl-tRNA formyltransferase (fmt) CSB-YP744842MLA
CSB-EP744842MLA
CSB-BP744842MLA
CSB-MP744842MLA
CSB-EP744842MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor P (efp) CSB-YP744843MLA
CSB-EP744843MLA
CSB-BP744843MLA
CSB-MP744843MLA
CSB-EP744843MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP744844MLA
CSB-EP744844MLA
CSB-BP744844MLA
CSB-MP744844MLA
CSB-EP744844MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP744845MLA
CSB-EP744845MLA
CSB-BP744845MLA
CSB-MP744845MLA
CSB-EP744845MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP744846MLA
CSB-EP744846MLA
CSB-BP744846MLA
CSB-MP744846MLA
CSB-EP744846MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycothiol acetyltransferase (mshD) CSB-YP744847MLA
CSB-EP744847MLA
CSB-BP744847MLA
CSB-MP744847MLA
CSB-EP744847MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cysteine--tRNA ligase (cysS) CSB-YP744848MLA
CSB-EP744848MLA
CSB-BP744848MLA
CSB-MP744848MLA
CSB-EP744848MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (Ile)-lysidine synthase (tilS) CSB-YP744849MLA
CSB-EP744849MLA
CSB-BP744849MLA
CSB-MP744849MLA
CSB-EP744849MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S18 1 (rpsR1) CSB-YP744850MLA
CSB-EP744850MLA
CSB-BP744850MLA
CSB-MP744850MLA
CSB-EP744850MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 5'-nucleotidase surE 2 (surE2) CSB-YP744851TNS
CSB-EP744851TNS
CSB-BP744851TNS
CSB-MP744851TNS
CSB-EP744851TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens NADH-quinone oxidoreductase subunit H 2 (nuoH2), partial CSB-YP744852GBK
CSB-EP744852GBK
CSB-BP744852GBK
CSB-MP744852GBK
CSB-EP744852GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP744853GBK
CSB-EP744853GBK
CSB-BP744853GBK
CSB-MP744853GBK
CSB-EP744853GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Adenylosuccinate synthetase (purA) CSB-YP744854GBK
CSB-EP744854GBK
CSB-BP744854GBK
CSB-MP744854GBK
CSB-EP744854GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 50S ribosomal protein L21 (rplU) CSB-YP744855GBK
CSB-EP744855GBK
CSB-BP744855GBK
CSB-MP744855GBK
CSB-EP744855GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP744856GBK
CSB-EP744856GBK
CSB-BP744856GBK
CSB-MP744856GBK
CSB-EP744856GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Thymidylate synthase thyX (thyX) CSB-YP744857GBK
CSB-EP744857GBK
CSB-BP744857GBK
CSB-MP744857GBK
CSB-EP744857GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Cell division protein FtsL (ftsL), partial CSB-YP744858GBK
CSB-EP744858GBK
CSB-BP744858GBK
CSB-MP744858GBK
CSB-EP744858GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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