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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Picrophilus torridus Digeranylgeranylglycerophospholipid reductase (PTO0896) CSB-YP743657PAAB
CSB-EP743657PAAB
CSB-BP743657PAAB
CSB-MP743657PAAB
CSB-EP743657PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 50S ribosomal protein L15e (rpl15e) CSB-YP743658PAAB
CSB-EP743658PAAB
CSB-BP743658PAAB
CSB-MP743658PAAB
CSB-EP743658PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 30S ribosomal protein S12P (rps12p) CSB-YP743659PAAB
CSB-EP743659PAAB
CSB-BP743659PAAB
CSB-MP743659PAAB
CSB-EP743659PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Probable DNA primase small subunit (priA) CSB-YP743660PAAB
CSB-EP743660PAAB
CSB-BP743660PAAB
CSB-MP743660PAAB
CSB-EP743660PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP743661PAAB
CSB-EP743661PAAB
CSB-BP743661PAAB
CSB-MP743661PAAB
CSB-EP743661PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 30S ribosomal protein S5P (rps5p) CSB-YP743662PAAB
CSB-EP743662PAAB
CSB-BP743662PAAB
CSB-MP743662PAAB
CSB-EP743662PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 50S ribosomal protein L2P (rpl2p) CSB-YP743663PAAB
CSB-EP743663PAAB
CSB-BP743663PAAB
CSB-MP743663PAAB
CSB-EP743663PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Probable spermidine synthase (speE) CSB-YP743664PAAB
CSB-EP743664PAAB
CSB-BP743664PAAB
CSB-MP743664PAAB
CSB-EP743664PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Putative HTH-type transcriptional regulatory protein PTO0557 (PTO0557) CSB-YP743665PAAB
CSB-EP743665PAAB
CSB-BP743665PAAB
CSB-MP743665PAAB
CSB-EP743665PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 30S ribosomal protein S2P (rps2p) CSB-YP743666PAAB
CSB-EP743666PAAB
CSB-BP743666PAAB
CSB-MP743666PAAB
CSB-EP743666PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP743667PAAB
CSB-EP743667PAAB
CSB-BP743667PAAB
CSB-MP743667PAAB
CSB-EP743667PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 7-cyano-7-deazaguanine tRNA-ribosyltransferase (tgtA), partial CSB-YP743668PAAB
CSB-EP743668PAAB
CSB-BP743668PAAB
CSB-MP743668PAAB
CSB-EP743668PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Alanine--tRNA ligase (alaS), partial CSB-YP743669PAAB
CSB-EP743669PAAB
CSB-BP743669PAAB
CSB-MP743669PAAB
CSB-EP743669PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Probable peroxiredoxin 1 (PTO0377) CSB-YP743670PAAB
CSB-EP743670PAAB
CSB-BP743670PAAB
CSB-MP743670PAAB
CSB-EP743670PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 50S ribosomal protein L13P (rpl13p) CSB-YP743671PAAB
CSB-EP743671PAAB
CSB-BP743671PAAB
CSB-MP743671PAAB
CSB-EP743671PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP743672PAAB
CSB-EP743672PAAB
CSB-BP743672PAAB
CSB-MP743672PAAB
CSB-EP743672PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum hybrid 30S ribosomal protein S8, chloroplastic (rps8) CSB-YP743673SAN
CSB-EP743673SAN
CSB-BP743673SAN
CSB-MP743673SAN
CSB-EP743673SAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum hybrid Photosystem II reaction center protein H (psbH), partial CSB-YP743674SAN1
CSB-EP743674SAN1
CSB-BP743674SAN1
CSB-MP743674SAN1
CSB-EP743674SAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum hybrid 50S ribosomal protein L33, chloroplastic (rpl33) CSB-YP743675SAN
CSB-EP743675SAN
CSB-BP743675SAN
CSB-MP743675SAN
CSB-EP743675SAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharum hybrid Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP743676SAN1
CSB-EP743676SAN1
CSB-BP743676SAN1
CSB-MP743676SAN1
CSB-EP743676SAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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