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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Agrobacterium tumefaciens Indole-3-glycerol phosphate synthase (trpC) CSB-YP745259AYS
CSB-EP745259AYS
CSB-BP745259AYS
CSB-MP745259AYS
CSB-EP745259AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP745260AYS
CSB-EP745260AYS
CSB-BP745260AYS
CSB-MP745260AYS
CSB-EP745260AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative conjugal transfer protein Rv3659c/MT3759 (Rv3659c, MT3759) CSB-YP745261MVZ
CSB-EP745261MVZ
CSB-BP745261MVZ
CSB-MP745261MVZ
CSB-EP745261MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized PPE family protein PPE51 (ppe51) CSB-YP745262MVZ
CSB-EP745262MVZ
CSB-BP745262MVZ
CSB-MP745262MVZ
CSB-EP745262MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative trans-acting enoyl reductase MT2525 (MT2525), partial CSB-YP745263MVZ1
CSB-EP745263MVZ1
CSB-BP745263MVZ1
CSB-MP745263MVZ1
CSB-EP745263MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative succinyl-diaminopimelate desuccinylase DapE (dapE) CSB-YP745264MVZ
CSB-EP745264MVZ
CSB-BP745264MVZ
CSB-MP745264MVZ
CSB-EP745264MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable sensor histidine kinase HK (MT0630) CSB-YP745265MVZ
CSB-EP745265MVZ
CSB-BP745265MVZ
CSB-MP745265MVZ
CSB-EP745265MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein ylbG (ylbG) CSB-YP745266EOD
CSB-EP745266EOD
CSB-BP745266EOD
CSB-MP745266EOD
CSB-EP745266EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP745267NGG
CSB-EP745267NGG
CSB-BP745267NGG
CSB-MP745267NGG
CSB-EP745267NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP745268NGG
CSB-EP745268NGG
CSB-BP745268NGG
CSB-MP745268NGG
CSB-EP745268NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B 50S ribosomal protein L16 (rplP) CSB-YP745269NGG
CSB-EP745269NGG
CSB-BP745269NGG
CSB-MP745269NGG
CSB-EP745269NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Oxygen-evolving enhancer protein 2-1, chloroplastic (PSBP1) CSB-YP745270NHE
CSB-EP745270NHE
CSB-BP745270NHE
CSB-MP745270NHE
CSB-EP745270NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Chitinase 2 (Cht2) CSB-YP745271OFG
CSB-EP745271OFG
CSB-BP745271OFG
CSB-MP745271OFG
CSB-EP745271OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica NAC domain-containing protein 67 (NAC67) CSB-YP745272OFG
CSB-EP745272OFG
CSB-BP745272OFG
CSB-MP745272OFG
CSB-EP745272OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Mitochondrial outer membrane protein porin 3 (VDAC3) CSB-YP745273OFG
CSB-EP745273OFG
CSB-BP745273OFG
CSB-MP745273OFG
CSB-EP745273OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Tricin synthase 2 (ROMT-17) CSB-YP745274OFG
CSB-EP745274OFG
CSB-BP745274OFG
CSB-MP745274OFG
CSB-EP745274OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 (GLO3) CSB-YP745275OFG
CSB-EP745275OFG
CSB-BP745275OFG
CSB-MP745275OFG
CSB-EP745275OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative ABC transporter C family member 15 (ABCC15), partial CSB-YP745276DOA
CSB-EP745276DOA
CSB-BP745276DOA
CSB-MP745276DOA
CSB-EP745276DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Atropa belladonna Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP745277AQX1
CSB-EP745277AQX1
CSB-BP745277AQX1
CSB-MP745277AQX1
CSB-EP745277AQX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Atropa belladonna 30S ribosomal protein S2, chloroplastic (rps2) CSB-YP745278AQX
CSB-EP745278AQX
CSB-BP745278AQX
CSB-MP745278AQX
CSB-EP745278AQX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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