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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus laevis SAM domain and HD domain-containing protein 1 (samhd1), partial CSB-YP743577XBE
CSB-EP743577XBE
CSB-BP743577XBE
CSB-MP743577XBE
CSB-EP743577XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Solute carrier family 25 member 46-A (slc25a46-a), partial CSB-YP743578XBE1
CSB-EP743578XBE1
CSB-BP743578XBE1
CSB-MP743578XBE1
CSB-EP743578XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Leucine-rich repeat-containing protein 57 (lrrc57) CSB-YP743579XBE
CSB-EP743579XBE
CSB-BP743579XBE
CSB-MP743579XBE
CSB-EP743579XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Serine/threonine-protein kinase 3 (stk3) CSB-YP743580XBE
CSB-EP743580XBE
CSB-BP743580XBE
CSB-MP743580XBE
CSB-EP743580XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Choline transporter-like protein 2 (slc44a2), partial CSB-YP743581XBE
CSB-EP743581XBE
CSB-BP743581XBE
CSB-MP743581XBE
CSB-EP743581XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nascent polypeptide-associated complex subunit alpha (naca) CSB-YP743582XBE
CSB-EP743582XBE
CSB-BP743582XBE
CSB-MP743582XBE
CSB-EP743582XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Serine/threonine-protein phosphatase 4 catalytic subunit (ppp4c) CSB-YP743583XBE
CSB-EP743583XBE
CSB-BP743583XBE
CSB-MP743583XBE
CSB-EP743583XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein C1orf96 (C1orf96) CSB-YP743584HU
CSB-EP743584HU
CSB-BP743584HU
CSB-MP743584HU
CSB-EP743584HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Transmembrane protein 8C (tmem8c), partial CSB-YP743585DIL1
CSB-EP743585DIL1
CSB-BP743585DIL1
CSB-MP743585DIL1
CSB-EP743585DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Calumenin-A (calua) CSB-YP743586DIL
CSB-EP743586DIL
CSB-BP743586DIL
CSB-MP743586DIL
CSB-EP743586DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Ribosome production factor 1 (rpf1) CSB-YP743587DIL
CSB-EP743587DIL
CSB-BP743587DIL
CSB-MP743587DIL
CSB-EP743587DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Zinc finger CW-type PWWP domain protein 1 (Zcwpw1) CSB-YP743588MO
CSB-EP743588MO
CSB-BP743588MO
CSB-MP743588MO
CSB-EP743588MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis TIP41-like protein (tiprl) CSB-YP743589XBE
CSB-EP743589XBE
CSB-BP743589XBE
CSB-MP743589XBE
CSB-EP743589XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Carboxypeptidase Q (Cpq) CSB-YP743590RA
CSB-EP743590RA
CSB-BP743590RA
CSB-MP743590RA
CSB-EP743590RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Hepatitis B virus X-interacting protein homolog (hbxip) CSB-YP743591XBE
CSB-EP743591XBE
CSB-BP743591XBE
CSB-MP743591XBE
CSB-EP743591XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative polypeptide N-acetylgalactosaminyltransferase-like protein 3 (WBSCR17), partial CSB-YP743592HU
CSB-EP743592HU
CSB-BP743592HU
CSB-MP743592HU
CSB-EP743592HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropidechis carinatus Protease inhibitor carinatin-1 CSB-YP743593TQQ
CSB-EP743593TQQ
CSB-BP743593TQQ
CSB-MP743593TQQ
CSB-EP743593TQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C), partial CSB-YP743594PI
CSB-EP743594PI
CSB-BP743594PI
CSB-MP743594PI
CSB-EP743594PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1 (Sohlh1) CSB-YP743595MO
CSB-EP743595MO
CSB-BP743595MO
CSB-MP743595MO
CSB-EP743595MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Gallinacin-11 (GAL11) CSB-YP743596CH
CSB-BP743596CH
CSB-MP743596CH
CSB-EP743596CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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