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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bdellovibrio bacteriovorus Enolase (eno) CSB-YP743857BFP
CSB-EP743857BFP
CSB-BP743857BFP
CSB-MP743857BFP
CSB-EP743857BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP743858BFP
CSB-EP743858BFP
CSB-BP743858BFP
CSB-MP743858BFP
CSB-EP743858BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus 50S ribosomal protein L31 type B (rpmE2) CSB-YP743859BFP
CSB-EP743859BFP
CSB-BP743859BFP
CSB-MP743859BFP
CSB-EP743859BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus 50S ribosomal protein L9 (rplI) CSB-YP743860BFP
CSB-EP743860BFP
CSB-BP743860BFP
CSB-MP743860BFP
CSB-EP743860BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L9 (rplI) CSB-YP743861MLS
CSB-EP743861MLS
CSB-BP743861MLS
CSB-MP743861MLS
CSB-EP743861MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC ATP synthase subunit b (atpF), partial CSB-YP743862MLS1
CSB-EP743862MLS1
CSB-BP743862MLS1
CSB-MP743862MLS1
CSB-EP743862MLS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 30S ribosomal protein S19 (rpsS) CSB-YP743863MLS
CSB-EP743863MLS
CSB-BP743863MLS
CSB-MP743863MLS
CSB-EP743863MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 30S ribosomal protein S8 (rpsH) CSB-YP743864MLS
CSB-EP743864MLS
CSB-BP743864MLS
CSB-MP743864MLS
CSB-EP743864MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 30S ribosomal protein S5 (rpsE) CSB-YP743865MLS
CSB-EP743865MLS
CSB-BP743865MLS
CSB-MP743865MLS
CSB-EP743865MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Adenylate kinase (adk) CSB-YP743866MLS
CSB-EP743866MLS
CSB-BP743866MLS
CSB-MP743866MLS
CSB-EP743866MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Energy-coupling factor transporter ATP-binding protein EcfA 2 (ecfA2) CSB-YP743867MLS
CSB-EP743867MLS
CSB-BP743867MLS
CSB-MP743867MLS
CSB-EP743867MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Chaperone protein DnaK (dnaK), partial CSB-YP743868MLS
CSB-EP743868MLS
CSB-BP743868MLS
CSB-MP743868MLS
CSB-EP743868MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP743869MLS
CSB-EP743869MLS
CSB-BP743869MLS
CSB-MP743869MLS
CSB-EP743869MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Glycine--tRNA ligase (glyQS) CSB-YP743870MLS
CSB-EP743870MLS
CSB-BP743870MLS
CSB-MP743870MLS
CSB-EP743870MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP743871MLS
CSB-EP743871MLS
CSB-BP743871MLS
CSB-MP743871MLS
CSB-EP743871MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Elongation factor 4 (lepA), partial CSB-YP743872MLS
CSB-EP743872MLS
CSB-BP743872MLS
CSB-MP743872MLS
CSB-EP743872MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Putative Holliday junction resolvase (MSC_0255) CSB-YP743873MLS
CSB-EP743873MLS
CSB-BP743873MLS
CSB-MP743873MLS
CSB-EP743873MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Release factor glutamine methyltransferase (prmC) CSB-YP743874MLS
CSB-EP743874MLS
CSB-BP743874MLS
CSB-MP743874MLS
CSB-EP743874MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Protein translocase subunit SecA (secA), partial CSB-YP743875MLS
CSB-EP743875MLS
CSB-BP743875MLS
CSB-MP743875MLS
CSB-EP743875MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Serine--tRNA ligase (serS) CSB-YP743876MLS
CSB-EP743876MLS
CSB-BP743876MLS
CSB-MP743876MLS
CSB-EP743876MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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