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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanococcus maripaludis DNA-directed RNA polymerase subunit A' (rpoA2) CSB-YP743777MSB
CSB-EP743777MSB
CSB-BP743777MSB
CSB-MP743777MSB
CSB-EP743777MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L18e (rpl18e) CSB-YP743778MSB
CSB-EP743778MSB
CSB-BP743778MSB
CSB-MP743778MSB
CSB-EP743778MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S13P (rps13p) CSB-YP743779MSB
CSB-EP743779MSB
CSB-BP743779MSB
CSB-MP743779MSB
CSB-EP743779MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable glucose-6-phosphate isomerase (pgi) CSB-YP743780MSB
CSB-EP743780MSB
CSB-BP743780MSB
CSB-MP743780MSB
CSB-EP743780MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP743781MSB
CSB-EP743781MSB
CSB-BP743781MSB
CSB-MP743781MSB
CSB-EP743781MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis L-fuculose phosphate aldolase (fucA) CSB-YP743782MSB
CSB-EP743782MSB
CSB-BP743782MSB
CSB-MP743782MSB
CSB-EP743782MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Cysteine--tRNA ligase (cysS) CSB-YP743783MSB
CSB-EP743783MSB
CSB-BP743783MSB
CSB-MP743783MSB
CSB-EP743783MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis V-type ATP synthase subunit F (atpF) CSB-YP743784MSB
CSB-EP743784MSB
CSB-BP743784MSB
CSB-MP743784MSB
CSB-EP743784MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis D-tyrosyl-tRNA (Tyr) deacylase (dtdA) CSB-YP743785MSB
CSB-EP743785MSB
CSB-BP743785MSB
CSB-MP743785MSB
CSB-EP743785MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Shikimate dehydrogenase (aroE) CSB-YP743786MSB
CSB-EP743786MSB
CSB-BP743786MSB
CSB-MP743786MSB
CSB-EP743786MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribonuclease Z (rnz) CSB-YP743787MSB
CSB-EP743787MSB
CSB-BP743787MSB
CSB-MP743787MSB
CSB-EP743787MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UDP-N-acetyl-D-mannosamine dehydrogenase (wecC) CSB-YP743788MSB
CSB-EP743788MSB
CSB-BP743788MSB
CSB-MP743788MSB
CSB-EP743788MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase (MMP0686) CSB-YP743789MSB
CSB-EP743789MSB
CSB-BP743789MSB
CSB-MP743789MSB
CSB-EP743789MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S3Ae (rps3Ae) CSB-YP743790MSB
CSB-EP743790MSB
CSB-BP743790MSB
CSB-MP743790MSB
CSB-EP743790MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Cytidylate kinase (cmk) CSB-YP743791MSB
CSB-EP743791MSB
CSB-BP743791MSB
CSB-MP743791MSB
CSB-EP743791MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase (flpA) CSB-YP743792MSB
CSB-EP743792MSB
CSB-BP743792MSB
CSB-MP743792MSB
CSB-EP743792MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Dihydrodipicolinate synthase (dapA) CSB-YP743793MSB
CSB-EP743793MSB
CSB-BP743793MSB
CSB-MP743793MSB
CSB-EP743793MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Digeranylgeranylglycerophospholipid reductase (MMP0388) CSB-YP743794MSB
CSB-EP743794MSB
CSB-BP743794MSB
CSB-MP743794MSB
CSB-EP743794MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Dihydroxy-acid dehydratase (ilvD), partial CSB-YP743795MSB
CSB-EP743795MSB
CSB-BP743795MSB
CSB-MP743795MSB
CSB-EP743795MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0248 protein MMP0286 (MMP0286) CSB-YP743796MSB
CSB-EP743796MSB
CSB-BP743796MSB
CSB-MP743796MSB
CSB-EP743796MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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