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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus laevis RNA polymerase II subunit A C-terminal domain phosphatase SSU72 (ssu72) CSB-YP743977XBE
CSB-EP743977XBE
CSB-BP743977XBE
CSB-MP743977XBE
CSB-EP743977XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis COP9 signalosome complex subunit 1 (csn1) CSB-YP743978XBE
CSB-EP743978XBE
CSB-BP743978XBE
CSB-MP743978XBE
CSB-EP743978XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Uncharacterized protein C15orf41 homolog CSB-YP743979XBE
CSB-EP743979XBE
CSB-BP743979XBE
CSB-MP743979XBE
CSB-EP743979XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Threonylcarbamoyladenosine tRNA methylthiotransferase (cdkal1), partial CSB-YP743980XBE1
CSB-EP743980XBE1
CSB-BP743980XBE1
CSB-MP743980XBE1
CSB-EP743980XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cancer/testis antigen family 45 member A5 (CT45A5) CSB-YP743981HU
CSB-EP743981HU
CSB-BP743981HU
CSB-MP743981HU
CSB-EP743981HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Leucine-rich repeat-containing protein 8E (LRRC8E), partial CSB-YP743982HU1
CSB-EP743982HU1
CSB-BP743982HU1
CSB-MP743982HU1
CSB-EP743982HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein LTV1 homolog (Ltv1) CSB-YP743983MO
CSB-EP743983MO
CSB-BP743983MO
CSB-MP743983MO
CSB-EP743983MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Carboxylesterase 5A (CES5A) CSB-YP743984HU
CSB-EP743984HU
CSB-BP743984HU
CSB-MP743984HU
CSB-EP743984HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Rhomboid domain-containing protein 2 (RHBDD2), partial CSB-YP743985HU1
CSB-EP743985HU1
CSB-BP743985HU1
CSB-MP743985HU1
CSB-EP743985HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Barrier-to-autointegration factor A (banf1-a) CSB-YP743986XBE
CSB-EP743986XBE
CSB-BP743986XBE
CSB-MP743986XBE
CSB-EP743986XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Tudor domain-containing protein 7 (tdrd7), partial CSB-YP743987XBE
CSB-EP743987XBE
CSB-BP743987XBE
CSB-MP743987XBE
CSB-EP743987XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein SPT2 homolog (spty2d1), partial CSB-YP743988XBE
CSB-EP743988XBE
CSB-BP743988XBE
CSB-MP743988XBE
CSB-EP743988XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis RNA polymerase II-associated protein 3 (rpap3), partial CSB-YP743989XBE
CSB-EP743989XBE
CSB-BP743989XBE
CSB-MP743989XBE
CSB-EP743989XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein CWC15 homolog A (cwc15-a) CSB-YP743990XBE
CSB-EP743990XBE
CSB-BP743990XBE
CSB-MP743990XBE
CSB-EP743990XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Charged multivesicular body protein 1a (chmp1a) CSB-YP743991XBE
CSB-EP743991XBE
CSB-BP743991XBE
CSB-MP743991XBE
CSB-EP743991XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Glycerol-3-phosphate acyltransferase 2, mitochondrial (GPAT2), partial CSB-YP743992HU1
CSB-EP743992HU1
CSB-BP743992HU1
CSB-MP743992HU1
CSB-EP743992HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Calcium-binding mitochondrial carrier protein SCaMC-1 (SLC25A24), partial CSB-YP743993HU1
CSB-EP743993HU1
CSB-BP743993HU1
CSB-MP743993HU1
CSB-EP743993HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Rab3 GTPase-activating protein catalytic subunit (rab3gap1), partial CSB-YP743994DIL
CSB-EP743994DIL
CSB-BP743994DIL
CSB-MP743994DIL
CSB-EP743994DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Acetolactate synthase-like protein (ilvbl), partial CSB-YP743995DIL
CSB-EP743995DIL
CSB-BP743995DIL
CSB-MP743995DIL
CSB-EP743995DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Mitochondrial Rho GTPase 1-A (rhot1a), partial CSB-YP743996DIL
CSB-EP743996DIL
CSB-BP743996DIL
CSB-MP743996DIL
CSB-EP743996DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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