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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Protochlamydia amoebophila Ribosomal RNA large subunit methyltransferase N 2 (rlmN2) CSB-YP743817PAAD
CSB-EP743817PAAD
CSB-BP743817PAAD
CSB-MP743817PAAD
CSB-EP743817PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP743818PAAD
CSB-EP743818PAAD
CSB-BP743818PAAD
CSB-MP743818PAAD
CSB-EP743818PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila UPF0337 protein pc0632 (pc0632) CSB-YP743819PAAD
CSB-EP743819PAAD
CSB-BP743819PAAD
CSB-MP743819PAAD
CSB-EP743819PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP743820PAAD
CSB-EP743820PAAD
CSB-BP743820PAAD
CSB-MP743820PAAD
CSB-EP743820PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 50S ribosomal protein L10 (rplJ) CSB-YP743821PAAD
CSB-EP743821PAAD
CSB-BP743821PAAD
CSB-MP743821PAAD
CSB-EP743821PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP743822PAAD
CSB-EP743822PAAD
CSB-BP743822PAAD
CSB-MP743822PAAD
CSB-EP743822PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Chaperone protein DnaJ (dnaJ) CSB-YP743823PAAD
CSB-EP743823PAAD
CSB-BP743823PAAD
CSB-MP743823PAAD
CSB-EP743823PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP743824PAAD
CSB-EP743824PAAD
CSB-BP743824PAAD
CSB-MP743824PAAD
CSB-EP743824PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 30S ribosomal protein S17 (rpsQ) CSB-YP743825PAAD
CSB-EP743825PAAD
CSB-BP743825PAAD
CSB-MP743825PAAD
CSB-EP743825PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 30S ribosomal protein S19 (rpsS) CSB-YP743826PAAD
CSB-EP743826PAAD
CSB-BP743826PAAD
CSB-MP743826PAAD
CSB-EP743826PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Nucleoside diphosphate kinase 1 (ndk1) CSB-YP743827PAAD
CSB-EP743827PAAD
CSB-BP743827PAAD
CSB-MP743827PAAD
CSB-EP743827PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP743828PAAD
CSB-EP743828PAAD
CSB-BP743828PAAD
CSB-MP743828PAAD
CSB-EP743828PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP743829PAAD
CSB-EP743829PAAD
CSB-BP743829PAAD
CSB-MP743829PAAD
CSB-EP743829PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Glutamate--tRNA ligase (gltX), partial CSB-YP743830PAAD
CSB-EP743830PAAD
CSB-BP743830PAAD
CSB-MP743830PAAD
CSB-EP743830PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 50S ribosomal protein L27 (rpmA) CSB-YP743831PAAD
CSB-EP743831PAAD
CSB-BP743831PAAD
CSB-MP743831PAAD
CSB-EP743831PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Enolase (eno) CSB-YP743832PAAD
CSB-EP743832PAAD
CSB-BP743832PAAD
CSB-MP743832PAAD
CSB-EP743832PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat DNA-directed RNA polymerase I subunit RPA12 (Znrd1) CSB-YP743833RA
CSB-EP743833RA
CSB-BP743833RA
CSB-MP743833RA
CSB-EP743833RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Nurim (Nrm), partial CSB-YP743834RA1
CSB-EP743834RA1
CSB-BP743834RA1
CSB-MP743834RA1
CSB-EP743834RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein G6b (G6b), partial CSB-YP743835RA
CSB-EP743835RA
CSB-BP743835RA
CSB-MP743835RA
CSB-EP743835RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat von Willebrand factor A domain-containing protein 7 (Vwa7), partial CSB-YP743836RA
CSB-EP743836RA
CSB-BP743836RA
CSB-MP743836RA
CSB-EP743836RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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