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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neurospora crassa Protein transport protein sec-24 (sec-24), partial CSB-YP745782NHA
CSB-EP745782NHA
CSB-BP745782NHA
CSB-MP745782NHA
CSB-EP745782NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Protoheme IX farnesyltransferase, mitochondrial (cox-10), partial CSB-YP745783NHA
CSB-EP745783NHA
CSB-BP745783NHA
CSB-MP745783NHA
CSB-EP745783NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Golgi apparatus membrane protein tvp-18 (tvp-18), partial CSB-YP745784NHA1
CSB-EP745784NHA1
CSB-BP745784NHA1
CSB-MP745784NHA1
CSB-EP745784NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa 40S ribosomal protein S28 (rps-28) CSB-YP745785NHA
CSB-EP745785NHA
CSB-BP745785NHA
CSB-MP745785NHA
CSB-EP745785NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Peptidyl-prolyl cis-trans isomerase B (cpr-2), partial CSB-YP745786NHA1
CSB-EP745786NHA1
CSB-BP745786NHA1
CSB-MP745786NHA1
CSB-EP745786NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Lysophospholipase nte-1 (nte-1), partial CSB-YP745787NHA
CSB-EP745787NHA
CSB-BP745787NHA
CSB-MP745787NHA
CSB-EP745787NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Pre-rRNA-processing protein esf-2 (esf-2) CSB-YP745788NHA
CSB-EP745788NHA
CSB-BP745788NHA
CSB-MP745788NHA
CSB-EP745788NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa RNA exonuclease 4 (rex-4) CSB-YP745789NHA
CSB-EP745789NHA
CSB-BP745789NHA
CSB-MP745789NHA
CSB-EP745789NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Eukaryotic translation initiation factor 3 subunit H (NCU07929) CSB-YP745790NHA
CSB-EP745790NHA
CSB-BP745790NHA
CSB-MP745790NHA
CSB-EP745790NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa DNA replication complex GINS protein psf-2 (psf-2) CSB-YP745791NHA
CSB-EP745791NHA
CSB-BP745791NHA
CSB-MP745791NHA
CSB-EP745791NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Vacuolar protein-sorting protein bro-1 (bro-1), partial CSB-YP745792NHA
CSB-EP745792NHA
CSB-BP745792NHA
CSB-MP745792NHA
CSB-EP745792NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa mRNA-capping enzyme subunit alpha (NCU06260) CSB-YP745793NHA
CSB-EP745793NHA
CSB-BP745793NHA
CSB-MP745793NHA
CSB-EP745793NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Increased rDNA silencing protein 4 (irs-4), partial CSB-YP745794NHA
CSB-EP745794NHA
CSB-BP745794NHA
CSB-MP745794NHA
CSB-EP745794NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Mitochondrial distribution and morphology protein 10 (mdm10) CSB-YP745795NHA
CSB-EP745795NHA
CSB-BP745795NHA
CSB-MP745795NHA
CSB-EP745795NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Probable tRNA-splicing endonuclease subunit sen-54 (sen-54), partial CSB-YP745796NHA
CSB-EP745796NHA
CSB-BP745796NHA
CSB-MP745796NHA
CSB-EP745796NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Protein sym-1 (sym-1), partial CSB-YP745797NHA1
CSB-EP745797NHA1
CSB-BP745797NHA1
CSB-MP745797NHA1
CSB-EP745797NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa ATP-dependent DNA helicase mph-1 (mph-1), partial CSB-YP745798NHA
CSB-EP745798NHA
CSB-BP745798NHA
CSB-MP745798NHA
CSB-EP745798NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Nascent polypeptide-associated complex subunit beta (egd-1) CSB-YP745799NHA
CSB-EP745799NHA
CSB-BP745799NHA
CSB-MP745799NHA
CSB-EP745799NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Sulfate adenylyltransferase (met-3), partial CSB-YP745800NHA
CSB-EP745800NHA
CSB-BP745800NHA
CSB-MP745800NHA
CSB-EP745800NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa pH-response regulator protein palC (palc-1) CSB-YP745801NHA
CSB-EP745801NHA
CSB-BP745801NHA
CSB-MP745801NHA
CSB-EP745801NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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