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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodopseudomonas palustris UPF0061 protein RPA3044 (RPA3044) CSB-YP743897RLU
CSB-EP743897RLU
CSB-BP743897RLU
CSB-MP743897RLU
CSB-EP743897RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP743898RLU
CSB-EP743898RLU
CSB-BP743898RLU
CSB-MP743898RLU
CSB-EP743898RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP743899RLU
CSB-EP743899RLU
CSB-BP743899RLU
CSB-MP743899RLU
CSB-EP743899RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 5'-nucleotidase surE (surE) CSB-YP743900RLU
CSB-EP743900RLU
CSB-BP743900RLU
CSB-MP743900RLU
CSB-EP743900RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 30S ribosomal protein S9 (rpsI) CSB-YP743901RLU
CSB-EP743901RLU
CSB-BP743901RLU
CSB-MP743901RLU
CSB-EP743901RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ribonuclease 3 (rnc) CSB-YP743902RLU
CSB-EP743902RLU
CSB-BP743902RLU
CSB-MP743902RLU
CSB-EP743902RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Aliphatic sulfonates import ATP-binding protein SsuB (ssuB) CSB-YP743903RLU
CSB-EP743903RLU
CSB-BP743903RLU
CSB-MP743903RLU
CSB-EP743903RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Putative 3-methyladenine DNA glycosylase (RPA2586) CSB-YP743904RLU
CSB-EP743904RLU
CSB-BP743904RLU
CSB-MP743904RLU
CSB-EP743904RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 2-isopropylmalate synthase (leuA), partial CSB-YP743905RLU
CSB-EP743905RLU
CSB-BP743905RLU
CSB-MP743905RLU
CSB-EP743905RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Pyrroloquinoline-quinone synthase (pqqC) CSB-YP743906RLU
CSB-EP743906RLU
CSB-BP743906RLU
CSB-MP743906RLU
CSB-EP743906RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP743907RLU
CSB-EP743907RLU
CSB-BP743907RLU
CSB-MP743907RLU
CSB-EP743907RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase (acsF) CSB-YP743908RLU
CSB-EP743908RLU
CSB-BP743908RLU
CSB-MP743908RLU
CSB-EP743908RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Methionine import ATP-binding protein MetN 1 (metN1) CSB-YP743909RLU
CSB-EP743909RLU
CSB-BP743909RLU
CSB-MP743909RLU
CSB-EP743909RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP743910RLU
CSB-EP743910RLU
CSB-BP743910RLU
CSB-MP743910RLU
CSB-EP743910RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP743911RLU
CSB-EP743911RLU
CSB-BP743911RLU
CSB-MP743911RLU
CSB-EP743911RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Flavoprotein WrbA (wrbA) CSB-YP743912RLU
CSB-EP743912RLU
CSB-BP743912RLU
CSB-MP743912RLU
CSB-EP743912RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP743913RLU
CSB-EP743913RLU
CSB-BP743913RLU
CSB-MP743913RLU
CSB-EP743913RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Recombination protein RecR (recR) CSB-YP743914RLU
CSB-EP743914RLU
CSB-BP743914RLU
CSB-MP743914RLU
CSB-EP743914RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Shikimate kinase (aroK) CSB-YP743915RLU
CSB-EP743915RLU
CSB-BP743915RLU
CSB-MP743915RLU
CSB-EP743915RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ribosome maturation factor RimP (rimP) CSB-YP743916RLU
CSB-EP743916RLU
CSB-BP743916RLU
CSB-MP743916RLU
CSB-EP743916RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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