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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Cleavage and polyadenylation specificity factor subunit 6 (Cpsf6) CSB-YP743997MO
CSB-EP743997MO
CSB-BP743997MO
CSB-MP743997MO
CSB-EP743997MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human ATPase SWSAP1 (SWSAP1) CSB-YP743998HU
CSB-EP743998HU
CSB-BP743998HU
CSB-MP743998HU
CSB-EP743998HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Histone H1.0 (h1f0) CSB-YP743999XBF
CSB-EP743999XBF
CSB-BP743999XBF
CSB-MP743999XBF
CSB-EP743999XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Iroquois-class homeodomain protein irx-3 (irx3) CSB-YP744000XBF
CSB-EP744000XBF
CSB-BP744000XBF
CSB-MP744000XBF
CSB-EP744000XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ergic3), partial CSB-YP744001XBF1
CSB-EP744001XBF1
CSB-BP744001XBF1
CSB-MP744001XBF1
CSB-EP744001XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Serine/threonine-protein phosphatase PP1-gamma catalytic subunit (ppp1cc) CSB-YP744002XBF
CSB-EP744002XBF
CSB-BP744002XBF
CSB-MP744002XBF
CSB-EP744002XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human RWD domain-containing protein 4 (RWDD4) CSB-YP744003HU
CSB-EP744003HU
CSB-BP744003HU
CSB-MP744003HU
CSB-EP744003HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Solute carrier family 2, facilitated glucose transporter member 12 (slc2a12), partial CSB-YP744004DIL
CSB-EP744004DIL
CSB-BP744004DIL
CSB-MP744004DIL
CSB-EP744004DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio DDB1- and CUL4-associated factor 10 (dcaf10), partial CSB-YP744005DIL
CSB-EP744005DIL
CSB-BP744005DIL
CSB-MP744005DIL
CSB-EP744005DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio AP-2 complex subunit mu-A (ap2m1a) CSB-YP744006DIL
CSB-EP744006DIL
CSB-BP744006DIL
CSB-MP744006DIL
CSB-EP744006DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Pre-mRNA-processing factor 40 homolog B (PRPF40B), partial CSB-YP744007HU
CSB-EP744007HU
CSB-BP744007HU
CSB-MP744007HU
CSB-EP744007HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Leucine-rich repeat-containing protein 59 (lrrc59), partial CSB-YP744008XBF
CSB-EP744008XBF
CSB-BP744008XBF
CSB-MP744008XBF
CSB-EP744008XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein dpy-19 homolog 2-like 1 (DPY19L2P1), partial CSB-YP744009HU1
CSB-EP744009HU1
CSB-BP744009HU1
CSB-MP744009HU1
CSB-EP744009HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc transporter 6 (SLC30A6), partial CSB-YP744010HU1
CSB-EP744010HU1
CSB-BP744010HU1
CSB-MP744010HU1
CSB-EP744010HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human KN motif and ankyrin repeat domain-containing protein 3 (KANK3), partial CSB-YP744011HU
CSB-EP744011HU
CSB-BP744011HU
CSB-MP744011HU
CSB-EP744011HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Large subunit GTPase 1 homolog (lsg1), partial CSB-YP744012DIL
CSB-EP744012DIL
CSB-BP744012DIL
CSB-MP744012DIL
CSB-EP744012DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio OCIA domain-containing protein 1 (ociad1) CSB-YP744013DIL
CSB-EP744013DIL
CSB-BP744013DIL
CSB-MP744013DIL
CSB-EP744013DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Intraflagellar transport protein 122 homolog (ift122), partial CSB-YP744014DIL
CSB-EP744014DIL
CSB-BP744014DIL
CSB-MP744014DIL
CSB-EP744014DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Kelch-like protein 21 (klhl21), partial CSB-YP744015DIL
CSB-EP744015DIL
CSB-BP744015DIL
CSB-MP744015DIL
CSB-EP744015DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Uracil phosphoribosyltransferase homolog (uprt) CSB-YP744016DIL
CSB-EP744016DIL
CSB-BP744016DIL
CSB-MP744016DIL
CSB-EP744016DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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