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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse APC membrane recruitment protein 1 (Fam123b), partial CSB-YP745882MO
CSB-EP745882MO
CSB-BP745882MO
CSB-MP745882MO
CSB-EP745882MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein PRRC2A (Prrc2a), partial CSB-YP745883MO
CSB-EP745883MO
CSB-BP745883MO
CSB-MP745883MO
CSB-EP745883MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse RNA polymerase II subunit A C-terminal domain phosphatase (Ctdp1), partial CSB-YP745884MO
CSB-BP745884MO
CSB-MP745884MO
CSB-EP745884MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Seizure protein 6 (Sez6), partial CSB-YP745885MO
CSB-EP745885MO
CSB-BP745885MO
CSB-MP745885MO
CSB-EP745885MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Immunoglobulin superfamily member 5 (Igsf5), partial CSB-YP745886MO
CSB-EP745886MO
CSB-BP745886MO
CSB-MP745886MO
CSB-EP745886MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Sterile alpha motif domain-containing protein 10 (Samd10) CSB-YP745887MO
CSB-EP745887MO
CSB-BP745887MO
CSB-MP745887MO
CSB-EP745887MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Putative aspartate aminotransferase, cytoplasmic 2 (Got1l1) CSB-YP745888MO
CSB-EP745888MO
CSB-BP745888MO
CSB-MP745888MO
CSB-EP745888MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Nucleus accumbens-associated protein 1 (Nacc1) CSB-YP745889MO
CSB-EP745889MO
CSB-BP745889MO
CSB-MP745889MO
CSB-EP745889MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Serine/threonine-protein kinase MRCK beta (Cdc42bpb), partial CSB-YP745890RA
CSB-EP745890RA
CSB-BP745890RA
CSB-MP745890RA
CSB-EP745890RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem Q (B) protein (psbA1), partial CSB-YP745891EYO1
CSB-EP745891EYO1
CSB-BP745891EYO1
CSB-MP745891EYO1
CSB-EP745891EYO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetaldehyde dehydrogenase (Mb3565c) CSB-YP745892MVH
CSB-EP745892MVH
CSB-BP745892MVH
CSB-MP745892MVH
CSB-EP745892MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP745893SSL
CSB-EP745893SSL
CSB-BP745893SSL
CSB-MP745893SSL
CSB-EP745893SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Bifunctional protein FolD (folD) CSB-YP745894SSL
CSB-EP745894SSL
CSB-BP745894SSL
CSB-MP745894SSL
CSB-EP745894SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Queuine tRNA-ribosyltransferase (tgt) CSB-YP745895SSL
CSB-EP745895SSL
CSB-BP745895SSL
CSB-MP745895SSL
CSB-EP745895SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Adenosylhomocysteinase (ahcY) CSB-YP745896RDR
CSB-EP745896RDR
CSB-BP745896RDR
CSB-MP745896RDR
CSB-EP745896RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 50S ribosomal protein L36 (rpmJ) CSB-YP745897EYQ
CSB-EP745897EYQ
CSB-BP745897EYQ
CSB-MP745897EYQ
CSB-EP745897EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP745898EYQ
CSB-EP745898EYQ
CSB-BP745898EYQ
CSB-MP745898EYQ
CSB-EP745898EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 30S ribosomal protein S15 (rpsO) CSB-YP745899EYQ
CSB-EP745899EYQ
CSB-BP745899EYQ
CSB-MP745899EYQ
CSB-EP745899EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Elongation factor Ts (tsf) CSB-YP745900EYQ
CSB-EP745900EYQ
CSB-BP745900EYQ
CSB-MP745900EYQ
CSB-EP745900EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Queuine tRNA-ribosyltransferase (tgt) CSB-YP745901EYQ
CSB-EP745901EYQ
CSB-BP745901EYQ
CSB-MP745901EYQ
CSB-EP745901EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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