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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Danio rerio CDGSH iron-sulfur domain-containing protein 2 (cisd2), partial CSB-YP745842DIL
CSB-EP745842DIL
CSB-BP745842DIL
CSB-MP745842DIL
CSB-EP745842DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Tumor necrosis factor, alpha-induced protein 8-like protein 2 B (tnfaip8l2b) CSB-YP745843DIL
CSB-EP745843DIL
CSB-BP745843DIL
CSB-MP745843DIL
CSB-EP745843DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Eukaryotic translation initiation factor 3 subunit M (eif3m) CSB-YP745844DIL
CSB-EP745844DIL
CSB-BP745844DIL
CSB-MP745844DIL
CSB-EP745844DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Kynurenine--oxoglutarate transaminase 3 (ccbl2) CSB-YP745845DIL
CSB-EP745845DIL
CSB-BP745845DIL
CSB-MP745845DIL
CSB-EP745845DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S ribosomal protein L15 (rpl15) CSB-YP745846SGAJ
CSB-EP745846SGAJ
CSB-BP745846SGAJ
CSB-MP745846SGAJ
CSB-EP745846SGAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anguilla japonica 60S ribosomal protein L15 (rpl15) CSB-YP745847BZH
CSB-EP745847BZH
CSB-BP745847BZH
CSB-MP745847BZH
CSB-EP745847BZH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Phosphatidylcholine:ceramide cholinephosphotransferase 1 (SGMS1), partial CSB-YP745848CH1
CSB-EP745848CH1
CSB-BP745848CH1
CSB-MP745848CH1
CSB-EP745848CH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine coronavirus Nucleoprotein (N) CSB-BP745849CHAH
CSB-MP745849CHAH
CSB-EP745849CHAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative serine/threonine-protein kinase/receptor R826 (MIMI_R826), partial CSB-YP745850ADAZ
CSB-EP745850ADAZ
CSB-BP745850ADAZ
CSB-MP745850ADAZ
CSB-EP745850ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Probable DNA polymerase sliding clamp (PCNA) CSB-YP745851ADAZ
CSB-EP745851ADAZ
CSB-BP745851ADAZ
CSB-MP745851ADAZ
CSB-EP745851ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Ribonucleoside-diphosphate reductase large subunit (RNR1), partial CSB-YP745852ADAZ
CSB-EP745852ADAZ
CSB-BP745852ADAZ
CSB-MP745852ADAZ
CSB-EP745852ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cestrum yellow leaf curling virus Uncharacterized protein 2 (ORF II) CSB-YP745853CHAI
CSB-EP745853CHAI
CSB-BP745853CHAI
CSB-MP745853CHAI
CSB-EP745853CHAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryphonectria parasitica mycoreovirus 1 RNA-directed RNA polymerase VP1, partial CSB-YP745854CFAT
CSB-EP745854CFAT
CSB-BP745854CFAT
CSB-MP745854CFAT
CSB-EP745854CFAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human SARS coronavirus Uncharacterized protein 14 (ORF14) CSB-YP745855HQE
CSB-EP745855HQE
CSB-BP745855HQE
CSB-MP745855HQE
CSB-EP745855HQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Lipid phosphate phosphatase-related protein type 3 (Lppr3), partial CSB-YP745856RA1
CSB-EP745856RA1
CSB-BP745856RA1
CSB-MP745856RA1
CSB-EP745856RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Desmoglein-4 (Dsg4), partial CSB-YP745857MO
CSB-EP745857MO
CSB-BP745857MO
CSB-MP745857MO
CSB-EP745857MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein Hook homolog 2 (Hook2) CSB-YP745858MO
CSB-EP745858MO
CSB-BP745858MO
CSB-MP745858MO
CSB-EP745858MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse ATP-binding cassette sub-family G member 2 (Abcg2), partial CSB-YP745859MO1
CSB-EP745859MO1
CSB-BP745859MO1
CSB-MP745859MO1
CSB-EP745859MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Mediator of RNA polymerase II transcription subunit 26 (Med26) CSB-YP745860MO
CSB-EP745860MO
CSB-BP745860MO
CSB-MP745860MO
CSB-EP745860MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Mas-related G-protein coupled receptor member B4 (Mrgprb4), partial CSB-YP745861RA1
CSB-EP745861RA1
CSB-BP745861RA1
CSB-MP745861RA1
CSB-EP745861RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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