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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acipenser baerii Keratin, type I cytoskeletal 18 (krt18) CSB-YP745822AAAJ
CSB-EP745822AAAJ
CSB-BP745822AAAJ
CSB-MP745822AAAJ
CSB-EP745822AAAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cytosolic 5'-nucleotidase 3 (nt5c3) CSB-YP745823DIL
CSB-EP745823DIL
CSB-BP745823DIL
CSB-MP745823DIL
CSB-EP745823DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis 40S ribosomal protein S8 (rps8) CSB-YP745824XBE
CSB-EP745824XBE
CSB-BP745824XBE
CSB-MP745824XBE
CSB-EP745824XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Small ubiquitin-related modifier 3 (sumo3) CSB-YP745825XBE
CSB-EP745825XBE
CSB-BP745825XBE
CSB-MP745825XBE
CSB-EP745825XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Charged multivesicular body protein 1b (chmp1b) CSB-YP745826XBE
CSB-EP745826XBE
CSB-BP745826XBE
CSB-MP745826XBE
CSB-EP745826XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Lethal (2) giant larvae protein homolog 2 (llgl2), partial CSB-YP745827DIL
CSB-EP745827DIL
CSB-BP745827DIL
CSB-MP745827DIL
CSB-EP745827DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis F-box-like/WD repeat-containing protein TBL1XR1-A (tbl1xr1-a), partial CSB-YP745828XBE
CSB-EP745828XBE
CSB-BP745828XBE
CSB-MP745828XBE
CSB-EP745828XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Mitochondrial fission factor homolog B (zgc:66022), partial CSB-YP745829DIL
CSB-EP745829DIL
CSB-BP745829DIL
CSB-MP745829DIL
CSB-EP745829DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Telomere length regulation protein TEL2 homolog (telo2), partial CSB-YP745830DIL
CSB-EP745830DIL
CSB-BP745830DIL
CSB-MP745830DIL
CSB-EP745830DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Gap junction Cx32.2 protein (cx32.2), partial CSB-YP745831DIL1
CSB-EP745831DIL1
CSB-BP745831DIL1
CSB-MP745831DIL1
CSB-EP745831DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis SH3 domain-binding glutamic acid-rich-like protein 2-A (sh3bgrl2-a) CSB-YP745832XBE
CSB-EP745832XBE
CSB-BP745832XBE
CSB-MP745832XBE
CSB-EP745832XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Matrix-remodeling-associated protein 8 (mxra8), partial CSB-YP745833XBE
CSB-EP745833XBE
CSB-BP745833XBE
CSB-MP745833XBE
CSB-EP745833XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ribosome biogenesis protein bop1-A (bop1-a), partial CSB-YP745834XBE
CSB-EP745834XBE
CSB-BP745834XBE
CSB-MP745834XBE
CSB-EP745834XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Vacuolar fusion protein CCZ1 homolog (ccz1) CSB-YP745835XBE
CSB-EP745835XBE
CSB-BP745835XBE
CSB-MP745835XBE
CSB-EP745835XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops jararacussu Zinc metalloproteinase BjussuMP-2 CSB-YP745836BUX
CSB-EP745836BUX
CSB-BP745836BUX
CSB-MP745836BUX
CSB-EP745836BUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Manganese-dependent ADP-ribose/CDP-alcohol diphosphatase (zgc:64213) CSB-YP745837DIL
CSB-EP745837DIL
CSB-BP745837DIL
CSB-MP745837DIL
CSB-EP745837DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio UPF0492 protein C20orf94 homolog (zgc:64090) CSB-YP745838DIL
CSB-EP745838DIL
CSB-BP745838DIL
CSB-MP745838DIL
CSB-EP745838DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelteobagrus fulvidraco 60S ribosomal protein L15 (rpl15) CSB-YP745839PHAA
CSB-EP745839PHAA
CSB-BP745839PHAA
CSB-MP745839PHAA
CSB-EP745839PHAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Echis multisquamatus EMS16 subunit A CSB-YP745840EAK
CSB-EP745840EAK
CSB-BP745840EAK
CSB-MP745840EAK
CSB-EP745840EAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio E3 ubiquitin-protein ligase RNF168 (rnf168) CSB-YP745841DIL
CSB-EP745841DIL
CSB-BP745841DIL
CSB-MP745841DIL
CSB-EP745841DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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