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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus Valine--tRNA ligase (valS), partial CSB-YP745902EYO
CSB-EP745902EYO
CSB-BP745902EYO
CSB-MP745902EYO
CSB-EP745902EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit H (ndhH) CSB-YP745903EYO
CSB-EP745903EYO
CSB-BP745903EYO
CSB-MP745903EYO
CSB-EP745903EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C (gatC) CSB-YP745904EYO
CSB-EP745904EYO
CSB-BP745904EYO
CSB-MP745904EYO
CSB-EP745904EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S20 (rpsT) CSB-YP745905EYO
CSB-EP745905EYO
CSB-BP745905EYO
CSB-MP745905EYO
CSB-EP745905EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Proline--tRNA ligase (proS), partial CSB-YP745906EYO
CSB-EP745906EYO
CSB-BP745906EYO
CSB-MP745906EYO
CSB-EP745906EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S15 (rpsO) CSB-YP745907EYO
CSB-EP745907EYO
CSB-BP745907EYO
CSB-MP745907EYO
CSB-EP745907EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Tryptophan synthase alpha chain (trpA) CSB-YP745908EYO
CSB-EP745908EYO
CSB-BP745908EYO
CSB-MP745908EYO
CSB-EP745908EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II 12 kDa extrinsic protein (psbU) CSB-YP745909EYO
CSB-EP745909EYO
CSB-BP745909EYO
CSB-MP745909EYO
CSB-EP745909EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 60 kDa chaperonin 1 (groL1), partial CSB-YP745910EYP
CSB-EP745910EYP
CSB-BP745910EYP
CSB-MP745910EYP
CSB-EP745910EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable long-chain-fatty-acid--CoA ligase FadD23 (fadD23), partial CSB-YP745911MVH1
CSB-EP745911MVH1
CSB-BP745911MVH1
CSB-MP745911MVH1
CSB-EP745911MVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative S-adenosyl-L-methionine-dependent methyltransferase Mb3816c (Mb3816c) CSB-YP745912MVH
CSB-EP745912MVH
CSB-BP745912MVH
CSB-MP745912MVH
CSB-EP745912MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized tRNA/rRNA methyltransferase Mb3610c (Mb3610c) CSB-YP745913MVH
CSB-EP745913MVH
CSB-BP745913MVH
CSB-MP745913MVH
CSB-EP745913MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Error-prone DNA polymerase (dnaE2), partial CSB-YP745914MVH
CSB-EP745914MVH
CSB-BP745914MVH
CSB-MP745914MVH
CSB-EP745914MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-phospho-L-lactate transferase (cofD) CSB-YP745915MVH
CSB-EP745915MVH
CSB-BP745915MVH
CSB-MP745915MVH
CSB-EP745915MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative thiosulfate sulfurtransferase 2 (cysA2) CSB-YP745916MVH
CSB-EP745916MVH
CSB-BP745916MVH
CSB-MP745916MVH
CSB-EP745916MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-alanine--D-alanine ligase (ddl) CSB-YP745917MVH
CSB-EP745917MVH
CSB-BP745917MVH
CSB-MP745917MVH
CSB-EP745917MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Long-chain-fatty-acid--AMP ligase FadD29 (fadD29), partial CSB-YP745918MVH
CSB-EP745918MVH
CSB-BP745918MVH
CSB-MP745918MVH
CSB-EP745918MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsB (ppsB), partial CSB-YP745919MVH
CSB-EP745919MVH
CSB-BP745919MVH
CSB-MP745919MVH
CSB-EP745919MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP745920MVH
CSB-EP745920MVH
CSB-BP745920MVH
CSB-MP745920MVH
CSB-EP745920MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trigger factor (tig) CSB-YP745921MVH
CSB-EP745921MVH
CSB-BP745921MVH
CSB-MP745921MVH
CSB-EP745921MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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