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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycoplasma mycoides subsp. mycoides SC tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG1), partial CSB-YP747204MLS
CSB-EP747204MLS
CSB-BP747204MLS
CSB-MP747204MLS
CSB-EP747204MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L1 (rplA) CSB-YP747205MLS
CSB-EP747205MLS
CSB-BP747205MLS
CSB-MP747205MLS
CSB-EP747205MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC ATP synthase subunit c (atpE), partial CSB-YP747206MLS1
CSB-EP747206MLS1
CSB-BP747206MLS1
CSB-MP747206MLS1
CSB-EP747206MLS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP747207MLS
CSB-EP747207MLS
CSB-BP747207MLS
CSB-MP747207MLS
CSB-EP747207MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L3 (rplC) CSB-YP747208MLS
CSB-EP747208MLS
CSB-BP747208MLS
CSB-MP747208MLS
CSB-EP747208MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L22 (rplV) CSB-YP747209MLS
CSB-EP747209MLS
CSB-BP747209MLS
CSB-MP747209MLS
CSB-EP747209MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L24 (rplX) CSB-YP747210MLS
CSB-EP747210MLS
CSB-BP747210MLS
CSB-MP747210MLS
CSB-EP747210MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Energy-coupling factor transporter ATP-binding protein EcfA 1 (ecfA1) CSB-YP747211MLS
CSB-EP747211MLS
CSB-BP747211MLS
CSB-MP747211MLS
CSB-EP747211MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Non-canonical purine NTP pyrophosphatase (MSC_0616) CSB-YP747212MLS
CSB-EP747212MLS
CSB-BP747212MLS
CSB-MP747212MLS
CSB-EP747212MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP747213MLS
CSB-EP747213MLS
CSB-BP747213MLS
CSB-MP747213MLS
CSB-EP747213MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC GTPase Der (der) CSB-YP747214MLS
CSB-EP747214MLS
CSB-BP747214MLS
CSB-MP747214MLS
CSB-EP747214MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC tRNA pseudouridine synthase B (truB) CSB-YP747215MLS
CSB-EP747215MLS
CSB-BP747215MLS
CSB-MP747215MLS
CSB-EP747215MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Transcription elongation factor GreA (greA) CSB-YP747216MLS
CSB-EP747216MLS
CSB-BP747216MLS
CSB-MP747216MLS
CSB-EP747216MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Phosphopantetheine adenylyltransferase (coaD) CSB-YP747217MLS
CSB-EP747217MLS
CSB-BP747217MLS
CSB-MP747217MLS
CSB-EP747217MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Spermidine/putrescine import ATP-binding protein PotA (potA) CSB-YP747218MLS
CSB-EP747218MLS
CSB-BP747218MLS
CSB-MP747218MLS
CSB-EP747218MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP747219MLS
CSB-EP747219MLS
CSB-BP747219MLS
CSB-MP747219MLS
CSB-EP747219MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC tRNA (Ile)-lysidine synthase (tilS) CSB-YP747220MLS
CSB-EP747220MLS
CSB-BP747220MLS
CSB-MP747220MLS
CSB-EP747220MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 30S ribosomal protein S18 (rpsR) CSB-YP747221MLS
CSB-EP747221MLS
CSB-BP747221MLS
CSB-MP747221MLS
CSB-EP747221MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Diphthine synthase (dph5) CSB-YP747222NGS
CSB-EP747222NGS
CSB-BP747222NGS
CSB-MP747222NGS
CSB-EP747222NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Regulator of G-protein signaling 7-binding protein (RGS7BP) CSB-YP747223HU
CSB-EP747223HU
CSB-BP747223HU
CSB-MP747223HU
CSB-EP747223HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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