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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodopseudomonas palustris Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP747264RLU
CSB-EP747264RLU
CSB-BP747264RLU
CSB-MP747264RLU
CSB-EP747264RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP747265RLU
CSB-EP747265RLU
CSB-BP747265RLU
CSB-MP747265RLU
CSB-EP747265RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Non-canonical purine NTP pyrophosphatase (RPA0328) CSB-YP747266RLU
CSB-EP747266RLU
CSB-BP747266RLU
CSB-MP747266RLU
CSB-EP747266RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris tRNA modification GTPase MnmE (mnmE) CSB-YP747267RLU
CSB-EP747267RLU
CSB-BP747267RLU
CSB-MP747267RLU
CSB-EP747267RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP747268RLU
CSB-EP747268RLU
CSB-BP747268RLU
CSB-MP747268RLU
CSB-EP747268RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris ATP synthase epsilon chain (atpC) CSB-YP747269RLU
CSB-EP747269RLU
CSB-BP747269RLU
CSB-MP747269RLU
CSB-EP747269RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP747270RLU
CSB-EP747270RLU
CSB-BP747270RLU
CSB-MP747270RLU
CSB-EP747270RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Cytidylate kinase (cmk) CSB-YP747271RLU
CSB-EP747271RLU
CSB-BP747271RLU
CSB-MP747271RLU
CSB-EP747271RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease P protein component (rnpA) CSB-YP747272CQF
CSB-EP747272CQF
CSB-BP747272CQF
CSB-MP747272CQF
CSB-EP747272CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP747273CQF
CSB-EP747273CQF
CSB-BP747273CQF
CSB-MP747273CQF
CSB-EP747273CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leucine--tRNA ligase (leuS), partial CSB-YP747274CQF
CSB-EP747274CQF
CSB-BP747274CQF
CSB-MP747274CQF
CSB-EP747274CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L9 (rplI) CSB-YP747275CQF
CSB-EP747275CQF
CSB-BP747275CQF
CSB-MP747275CQF
CSB-EP747275CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylosuccinate synthetase (purA) CSB-YP747276CQF
CSB-EP747276CQF
CSB-BP747276CQF
CSB-MP747276CQF
CSB-EP747276CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP747277CQF
CSB-EP747277CQF
CSB-BP747277CQF
CSB-MP747277CQF
CSB-EP747277CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycothiol acetyltransferase (mshD) CSB-YP747278CQF
CSB-EP747278CQF
CSB-BP747278CQF
CSB-MP747278CQF
CSB-EP747278CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease PH (rph) CSB-YP747279CQF
CSB-EP747279CQF
CSB-BP747279CQF
CSB-MP747279CQF
CSB-EP747279CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP747280CQF
CSB-EP747280CQF
CSB-BP747280CQF
CSB-MP747280CQF
CSB-EP747280CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gamma-glutamyl phosphate reductase (proA) CSB-YP747281CQF
CSB-EP747281CQF
CSB-BP747281CQF
CSB-MP747281CQF
CSB-EP747281CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deoxyguanosinetriphosphate triphosphohydrolase-like protein (DIP1703) CSB-YP747282CQF
CSB-EP747282CQF
CSB-BP747282CQF
CSB-MP747282CQF
CSB-EP747282CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable menaquinol-cytochrome c reductase cytochrome c subunit (qcrC), partial CSB-YP747283CQF1
CSB-EP747283CQF1
CSB-BP747283CQF1
CSB-MP747283CQF1
CSB-EP747283CQF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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