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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Thermus thermophilus Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP748004TNS
CSB-EP748004TNS
CSB-BP748004TNS
CSB-MP748004TNS
CSB-EP748004TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Octanoyltransferase (lipB) CSB-YP748005TNS
CSB-EP748005TNS
CSB-BP748005TNS
CSB-MP748005TNS
CSB-EP748005TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP748006TNS
CSB-EP748006TNS
CSB-BP748006TNS
CSB-MP748006TNS
CSB-EP748006TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 5'-nucleotidase surE 1 (surE1) CSB-YP748007TNS
CSB-EP748007TNS
CSB-BP748007TNS
CSB-MP748007TNS
CSB-EP748007TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Enolase (eno) CSB-YP748008TNS
CSB-EP748008TNS
CSB-BP748008TNS
CSB-MP748008TNS
CSB-EP748008TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Peptide chain release factor 1 (prfA) CSB-YP748009TNS
CSB-EP748009TNS
CSB-BP748009TNS
CSB-MP748009TNS
CSB-EP748009TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Anthranilate phosphoribosyltransferase (trpD) CSB-YP748010TNS
CSB-EP748010TNS
CSB-BP748010TNS
CSB-MP748010TNS
CSB-EP748010TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP748011TNS
CSB-EP748011TNS
CSB-BP748011TNS
CSB-MP748011TNS
CSB-EP748011TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP748012TNS
CSB-EP748012TNS
CSB-BP748012TNS
CSB-MP748012TNS
CSB-EP748012TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Pantothenate synthetase (panC) CSB-YP748013TNS
CSB-EP748013TNS
CSB-BP748013TNS
CSB-MP748013TNS
CSB-EP748013TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Elongation factor G (fusA), partial CSB-YP748014TNS
CSB-EP748014TNS
CSB-BP748014TNS
CSB-MP748014TNS
CSB-EP748014TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 50S ribosomal protein L18 (rplR) CSB-YP748015TNS
CSB-EP748015TNS
CSB-BP748015TNS
CSB-MP748015TNS
CSB-EP748015TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus UPF0133 protein TT_C1235 (TT_C1235) CSB-YP748016TNS
CSB-EP748016TNS
CSB-BP748016TNS
CSB-MP748016TNS
CSB-EP748016TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Arginine repressor (argR) CSB-YP748017TNS
CSB-EP748017TNS
CSB-BP748017TNS
CSB-MP748017TNS
CSB-EP748017TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP748018TNS
CSB-EP748018TNS
CSB-BP748018TNS
CSB-MP748018TNS
CSB-EP748018TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO (trmFO) CSB-YP748019TNS
CSB-EP748019TNS
CSB-BP748019TNS
CSB-MP748019TNS
CSB-EP748019TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP748020TNS
CSB-EP748020TNS
CSB-BP748020TNS
CSB-MP748020TNS
CSB-EP748020TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus V-type ATP synthase subunit C (atpC) CSB-YP748021TNS
CSB-EP748021TNS
CSB-BP748021TNS
CSB-MP748021TNS
CSB-EP748021TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Acetyl-coenzyme A synthetase (acsA), partial CSB-YP748022TNS
CSB-EP748022TNS
CSB-BP748022TNS
CSB-MP748022TNS
CSB-EP748022TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP748023TNS
CSB-EP748023TNS
CSB-BP748023TNS
CSB-MP748023TNS
CSB-EP748023TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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