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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vibrio vulnificus Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP748804VCQ
CSB-EP748804VCQ
CSB-BP748804VCQ
CSB-MP748804VCQ
CSB-EP748804VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Probable transcriptional regulatory protein VVA0010 (VVA0010) CSB-YP748805VCQ
CSB-EP748805VCQ
CSB-BP748805VCQ
CSB-MP748805VCQ
CSB-EP748805VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus ATP synthase subunit beta (atpD) CSB-YP748806VCQ
CSB-EP748806VCQ
CSB-BP748806VCQ
CSB-MP748806VCQ
CSB-EP748806VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Thiazole synthase (thiG) CSB-YP748807VCQ
CSB-EP748807VCQ
CSB-BP748807VCQ
CSB-MP748807VCQ
CSB-EP748807VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 50S ribosomal protein L1 (rplA) CSB-YP748808VCQ
CSB-EP748808VCQ
CSB-BP748808VCQ
CSB-MP748808VCQ
CSB-EP748808VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus DNA-binding protein fis (fis) CSB-YP748809VCQ
CSB-EP748809VCQ
CSB-BP748809VCQ
CSB-MP748809VCQ
CSB-EP748809VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Fumarate reductase subunit C (frdC), partial CSB-YP748810VCQ1
CSB-EP748810VCQ1
CSB-BP748810VCQ1
CSB-MP748810VCQ1
CSB-EP748810VCQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP748811VCQ
CSB-EP748811VCQ
CSB-BP748811VCQ
CSB-MP748811VCQ
CSB-EP748811VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus DNA mismatch repair protein mutL (mutL), partial CSB-YP748812VCQ
CSB-EP748812VCQ
CSB-BP748812VCQ
CSB-MP748812VCQ
CSB-EP748812VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP748813VCQ
CSB-EP748813VCQ
CSB-BP748813VCQ
CSB-MP748813VCQ
CSB-EP748813VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Cell division protein ZapB (zapB) CSB-YP748814VCQ
CSB-EP748814VCQ
CSB-BP748814VCQ
CSB-MP748814VCQ
CSB-EP748814VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Met repressor (metJ) CSB-YP748815VCQ
CSB-EP748815VCQ
CSB-BP748815VCQ
CSB-MP748815VCQ
CSB-EP748815VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 3-dehydroquinate synthase (aroB) CSB-YP748816VCQ
CSB-EP748816VCQ
CSB-BP748816VCQ
CSB-MP748816VCQ
CSB-EP748816VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP748817VCQ
CSB-EP748817VCQ
CSB-BP748817VCQ
CSB-MP748817VCQ
CSB-EP748817VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0235 protein VV2877 (VV2877) CSB-YP748818VCQ
CSB-EP748818VCQ
CSB-BP748818VCQ
CSB-MP748818VCQ
CSB-EP748818VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Transketolase 1 (tkt1), partial CSB-YP748819VCQ
CSB-EP748819VCQ
CSB-BP748819VCQ
CSB-MP748819VCQ
CSB-EP748819VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus GTPase Era (era) CSB-YP748820VCQ
CSB-EP748820VCQ
CSB-BP748820VCQ
CSB-MP748820VCQ
CSB-EP748820VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Enolase (eno) CSB-YP748821VCQ
CSB-EP748821VCQ
CSB-BP748821VCQ
CSB-MP748821VCQ
CSB-EP748821VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Regulatory protein recX (recX) CSB-YP748822VCQ
CSB-EP748822VCQ
CSB-BP748822VCQ
CSB-MP748822VCQ
CSB-EP748822VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribosome maturation factor RimM (rimM) CSB-YP748823VCQ
CSB-EP748823VCQ
CSB-BP748823VCQ
CSB-MP748823VCQ
CSB-EP748823VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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