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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 50S ribosomal protein L15 (rplO) CSB-YP747704SJV
CSB-EP747704SJV
CSB-BP747704SJV
CSB-MP747704SJV
CSB-EP747704SJV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L16 (rplP) CSB-YP747705SJV
CSB-EP747705SJV
CSB-BP747705SJV
CSB-MP747705SJV
CSB-EP747705SJV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Fructokinase-1 (FRK1) CSB-YP747706ZAX
CSB-EP747706ZAX
CSB-BP747706ZAX
CSB-MP747706ZAX
CSB-EP747706ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tachyglossus aculeatus aculeatus Interferon beta (IFNB1) CSB-YP747707TKU
CSB-EP747707TKU
CSB-BP747707TKU
CSB-MP747707TKU
CSB-EP747707TKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pagrus major SUMO-conjugating enzyme UBC9 (ube2i) CSB-YP747708EQR
CSB-EP747708EQR
CSB-BP747708EQR
CSB-MP747708EQR
CSB-EP747708EQR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Multidrug resistance-associated protein 9 (Abcc12), partial CSB-YP747709RA
CSB-EP747709RA
CSB-BP747709RA
CSB-MP747709RA
CSB-EP747709RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative ribonucleoside-diphosphate reductase small chain B (RNR2B) CSB-YP747710DOA
CSB-EP747710DOA
CSB-BP747710DOA
CSB-MP747710DOA
CSB-EP747710DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canis lupus Cytochrome b (MT-CYB), partial CSB-YP747711CCW
CSB-EP747711CCW
CSB-BP747711CCW
CSB-MP747711CCW
CSB-EP747711CCW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antirrhinum majus Protein WUSCHEL (WUS) CSB-YP747712DNE
CSB-EP747712DNE
CSB-BP747712DNE
CSB-MP747712DNE
CSB-EP747712DNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachancea kluyveri Monothiol glutaredoxin-5, mitochondrial (GRX5) CSB-YP747713LMA
CSB-EP747713LMA
CSB-BP747713LMA
CSB-MP747713LMA
CSB-EP747713LMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cytochrome c oxidase subunit 6B2 (COX6B2) CSB-YP747714HU
CSB-EP747714HU
CSB-BP747714HU
CSB-MP747714HU
CSB-EP747714HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyto alba Circadian locomoter output cycles protein kaput (CLOCK), partial CSB-YP747715TCAG
CSB-EP747715TCAG
CSB-BP747715TCAG
CSB-MP747715TCAG
CSB-EP747715TCAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sclerotinia sclerotiorum debilitation-associated virus RNA replication protein, partial CSB-YP747716SGAK
CSB-EP747716SGAK
CSB-BP747716SGAK
CSB-MP747716SGAK
CSB-EP747716SGAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus candidus Phospholipase A2, beta bungarotoxin A chain CSB-YP747717BPN
CSB-EP747717BPN
CSB-BP747717BPN
CSB-MP747717BPN
CSB-EP747717BPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 1 CSB-YP747718ROM
CSB-EP747718ROM
CSB-BP747718ROM
CSB-MP747718ROM
CSB-EP747718ROM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botrytis virus X Capsid protein (ORF3) CSB-YP747719BDAR
CSB-EP747719BDAR
CSB-BP747719BDAR
CSB-MP747719BDAR
CSB-EP747719BDAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monodelphis domestica Glycoprotein hormones alpha chain (CGA) CSB-YP747720MUE
CSB-EP747720MUE
CSB-BP747720MUE
CSB-MP747720MUE
CSB-EP747720MUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Serine/threonine-protein kinase UL13 homolog (ORF47), partial CSB-YP747721VAQ
CSB-EP747721VAQ
CSB-BP747721VAQ
CSB-MP747721VAQ
CSB-EP747721VAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Chaperone protein DnaJ (dnaJ) CSB-YP747722OBN
CSB-EP747722OBN
CSB-BP747722OBN
CSB-MP747722OBN
CSB-EP747722OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP747723OBN
CSB-EP747723OBN
CSB-BP747723OBN
CSB-MP747723OBN
CSB-EP747723OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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