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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Long-chain-fatty-acid--AMP ligase FadD26 (fadD26), partial CSB-YP749304MVH
CSB-EP749304MVH
CSB-BP749304MVH
CSB-MP749304MVH
CSB-EP749304MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0678 fatty acid-binding protein-like protein Mb2736c (Mb2736c) CSB-YP749305MVH
CSB-EP749305MVH
CSB-BP749305MVH
CSB-MP749305MVH
CSB-EP749305MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N utilization substance protein B homolog (nusB) CSB-YP749306MVH
CSB-EP749306MVH
CSB-BP749306MVH
CSB-MP749306MVH
CSB-EP749306MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Isochorismate synthase/isochorismate-pyruvate lyase mbtI (mbtI) CSB-YP749307MVH
CSB-EP749307MVH
CSB-BP749307MVH
CSB-MP749307MVH
CSB-EP749307MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prokaryotic ubiquitin-like protein Pup (pup) CSB-YP749308MVH
CSB-EP749308MVH
CSB-BP749308MVH
CSB-MP749308MVH
CSB-EP749308MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine cleavage system H protein (gcvH) CSB-YP749309MVH
CSB-EP749309MVH
CSB-BP749309MVH
CSB-MP749309MVH
CSB-EP749309MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine synthase (metK) CSB-YP749310MVH
CSB-EP749310MVH
CSB-BP749310MVH
CSB-MP749310MVH
CSB-EP749310MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative O-methyltransferase Mb1252c (Mb1252c) CSB-YP749311MVH
CSB-EP749311MVH
CSB-BP749311MVH
CSB-MP749311MVH
CSB-EP749311MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acyl-CoA dehydrogenase fadE12 (fadE12) CSB-YP749312MVH
CSB-EP749312MVH
CSB-BP749312MVH
CSB-MP749312MVH
CSB-EP749312MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative S-adenosyl-L-methionine-dependent methyltransferase Mb0853 (Mb0853) CSB-YP749313MVH
CSB-EP749313MVH
CSB-BP749313MVH
CSB-MP749313MVH
CSB-EP749313MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L15 (rplO) CSB-YP749314MVH
CSB-EP749314MVH
CSB-BP749314MVH
CSB-MP749314MVH
CSB-EP749314MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase sigma factor sigK (sigK) CSB-YP749315MVH
CSB-EP749315MVH
CSB-BP749315MVH
CSB-MP749315MVH
CSB-EP749315MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Succinate-semialdehyde dehydrogenase [NADP (+)] 1 (gabD1) CSB-YP749316MVH
CSB-EP749316MVH
CSB-BP749316MVH
CSB-MP749316MVH
CSB-EP749316MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0182 protein Mb0065 (Mb0065), partial CSB-YP749317MVH
CSB-EP749317MVH
CSB-BP749317MVH
CSB-MP749317MVH
CSB-EP749317MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP749318HCO
CSB-EP749318HCO
CSB-BP749318HCO
CSB-MP749318HCO
CSB-EP749318HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP749319HSY
CSB-EP749319HSY
CSB-BP749319HSY
CSB-MP749319HSY
CSB-EP749319HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA dimethylallyltransferase (miaA) CSB-YP749320BHM
CSB-EP749320BHM
CSB-BP749320BHM
CSB-MP749320BHM
CSB-EP749320BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis CCA-adding enzyme (cca) CSB-YP749321BUA
CSB-EP749321BUA
CSB-BP749321BUA
CSB-MP749321BUA
CSB-EP749321BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica HPr kinase/phosphorylase (hprK) CSB-YP749322BTY
CSB-EP749322BTY
CSB-BP749322BTY
CSB-MP749322BTY
CSB-EP749322BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP749323SSL
CSB-EP749323SSL
CSB-BP749323SSL
CSB-MP749323SSL
CSB-EP749323SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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