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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chromobacterium violaceum Glutamate--tRNA ligase (gltX) CSB-YP749104CKA
CSB-EP749104CKA
CSB-BP749104CKA
CSB-MP749104CKA
CSB-EP749104CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Sulfate/thiosulfate import ATP-binding protein CysA 1 (cysA1) CSB-YP749105CKA
CSB-EP749105CKA
CSB-BP749105CKA
CSB-MP749105CKA
CSB-EP749105CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Cysteine--tRNA ligase (cysS) CSB-YP749106CKA
CSB-EP749106CKA
CSB-BP749106CKA
CSB-MP749106CKA
CSB-EP749106CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Protein grpE (grpE) CSB-YP749107CKA
CSB-EP749107CKA
CSB-BP749107CKA
CSB-MP749107CKA
CSB-EP749107CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Recombination protein RecR (recR) CSB-YP749108CKA
CSB-EP749108CKA
CSB-BP749108CKA
CSB-MP749108CKA
CSB-EP749108CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP749109CKA
CSB-EP749109CKA
CSB-BP749109CKA
CSB-MP749109CKA
CSB-EP749109CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP749110CKA
CSB-EP749110CKA
CSB-BP749110CKA
CSB-MP749110CKA
CSB-EP749110CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Serine--tRNA ligase (serS) CSB-YP749111CKA
CSB-EP749111CKA
CSB-BP749111CKA
CSB-MP749111CKA
CSB-EP749111CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Translation initiation factor IF-3 (infC) CSB-YP749112CKA
CSB-EP749112CKA
CSB-BP749112CKA
CSB-MP749112CKA
CSB-EP749112CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Dihydroxy-acid dehydratase (ilvD), partial CSB-YP749113CKA
CSB-EP749113CKA
CSB-BP749113CKA
CSB-MP749113CKA
CSB-EP749113CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP749114CKA
CSB-EP749114CKA
CSB-BP749114CKA
CSB-MP749114CKA
CSB-EP749114CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Protein-export protein SecB (secB) CSB-YP749115CKA
CSB-EP749115CKA
CSB-BP749115CKA
CSB-MP749115CKA
CSB-EP749115CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Lysine--tRNA ligase (lysS), partial CSB-YP749116CKA
CSB-EP749116CKA
CSB-BP749116CKA
CSB-MP749116CKA
CSB-EP749116CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 3-dehydroquinate dehydratase (aroQ) CSB-YP749117CKA
CSB-EP749117CKA
CSB-BP749117CKA
CSB-MP749117CKA
CSB-EP749117CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum NADH-quinone oxidoreductase subunit B 1 (nuoB1) CSB-YP749118CKA
CSB-EP749118CKA
CSB-BP749118CKA
CSB-MP749118CKA
CSB-EP749118CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 3-dehydroquinate synthase (aroB) CSB-YP749119CKA
CSB-EP749119CKA
CSB-BP749119CKA
CSB-MP749119CKA
CSB-EP749119CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Adenylosuccinate synthetase 1 (purA1) CSB-YP749120CKA
CSB-EP749120CKA
CSB-BP749120CKA
CSB-MP749120CKA
CSB-EP749120CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP749121CKA
CSB-EP749121CKA
CSB-BP749121CKA
CSB-MP749121CKA
CSB-EP749121CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP749122CKA
CSB-EP749122CKA
CSB-BP749122CKA
CSB-MP749122CKA
CSB-EP749122CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Ketol-acid reductoisomerase (ilvC) CSB-YP749123CKA
CSB-EP749123CKA
CSB-BP749123CKA
CSB-MP749123CKA
CSB-EP749123CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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