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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Onion yellows phytoplasma Protein translocase subunit SecA (secA), partial CSB-YP747724OBN
CSB-EP747724OBN
CSB-BP747724OBN
CSB-MP747724OBN
CSB-EP747724OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Trigger factor (tig) CSB-YP747725OBN
CSB-EP747725OBN
CSB-BP747725OBN
CSB-MP747725OBN
CSB-EP747725OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Alanine--tRNA ligase (alaS), partial CSB-YP747726OBN
CSB-EP747726OBN
CSB-BP747726OBN
CSB-MP747726OBN
CSB-EP747726OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Ribonuclease Y (rny), partial CSB-YP747727OBN1
CSB-EP747727OBN1
CSB-BP747727OBN1
CSB-MP747727OBN1
CSB-EP747727OBN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 30S ribosomal protein S7 (rpsG) CSB-YP747728OBN
CSB-EP747728OBN
CSB-BP747728OBN
CSB-MP747728OBN
CSB-EP747728OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Proline--tRNA ligase (proS) CSB-YP747729OBN
CSB-EP747729OBN
CSB-BP747729OBN
CSB-MP747729OBN
CSB-EP747729OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP747730OBN
CSB-EP747730OBN
CSB-BP747730OBN
CSB-MP747730OBN
CSB-EP747730OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 50S ribosomal protein L6 (rplF) CSB-YP747731OBN
CSB-EP747731OBN
CSB-BP747731OBN
CSB-MP747731OBN
CSB-EP747731OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 30S ribosomal protein S15 (rpsO) CSB-YP747732OBN
CSB-EP747732OBN
CSB-BP747732OBN
CSB-MP747732OBN
CSB-EP747732OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 50S ribosomal protein L28 (rpmB) CSB-YP747733OBN
CSB-EP747733OBN
CSB-BP747733OBN
CSB-MP747733OBN
CSB-EP747733OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Valine--tRNA ligase (valS), partial CSB-YP747734OBN
CSB-EP747734OBN
CSB-BP747734OBN
CSB-MP747734OBN
CSB-EP747734OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Proteasome subunit alpha type-7-A (Os08g0548900, LOC_Os08g43540) CSB-YP747735OFG
CSB-EP747735OFG
CSB-BP747735OFG
CSB-MP747735OFG
CSB-EP747735OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative multidrug resistance protein (Os02g0190300, LOC_Os02g09720), partial CSB-YP747736OFG
CSB-EP747736OFG
CSB-BP747736OFG
CSB-MP747736OFG
CSB-EP747736OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Elongation factor 1-gamma 2 (Os02g0220500, LOC_Os02g12800) CSB-YP747737OFG
CSB-EP747737OFG
CSB-BP747737OFG
CSB-MP747737OFG
CSB-EP747737OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens 30S ribosomal protein S11, chloroplastic (rps11) CSB-YP747738EVD
CSB-EP747738EVD
CSB-BP747738EVD
CSB-MP747738EVD
CSB-EP747738EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens 30S ribosomal protein S19, chloroplastic (rps19) CSB-YP747739EVD
CSB-EP747739EVD
CSB-BP747739EVD
CSB-MP747739EVD
CSB-EP747739EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens 50S ribosomal protein L14, chloroplastic (rpl14) CSB-YP747740EVD
CSB-EP747740EVD
CSB-BP747740EVD
CSB-MP747740EVD
CSB-EP747740EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Cytochrome b559 subunit alpha (psbE), partial CSB-YP747741EVD1
CSB-EP747741EVD1
CSB-BP747741EVD1
CSB-MP747741EVD1
CSB-EP747741EVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Photosystem II reaction center protein H (psbH), partial CSB-YP747742EVD1
CSB-EP747742EVD1
CSB-BP747742EVD1
CSB-MP747742EVD1
CSB-EP747742EVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Maturase K (matK), partial CSB-YP747743EVD
CSB-EP747743EVD
CSB-BP747743EVD
CSB-MP747743EVD
CSB-EP747743EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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