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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus tropicalis cAMP-responsive element-binding protein-like 2 (crebl2) CSB-YP005955XBF
CSB-EP005955XBF
CSB-BP005955XBF
CSB-MP005955XBF
CSB-EP005955XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Methyltransferase-like protein 21A (mettl21a) CSB-YP008022XBF
CSB-EP008022XBF
CSB-BP008022XBF
CSB-MP008022XBF
CSB-EP008022XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Zinc transporter ZIP14 (slc39a14), partial CSB-YP021630XBF1
CSB-EP021630XBF1
CSB-BP021630XBF1
CSB-MP021630XBF1
CSB-EP021630XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis LSM domain-containing protein 1 (lsmd1) CSB-YP013213XBF
CSB-EP013213XBF
CSB-BP013213XBF
CSB-MP013213XBF
CSB-EP013213XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Autophagy-related protein 101 (atg101) CSB-YP003077XBF
CSB-EP003077XBF
CSB-BP003077XBF
CSB-MP003077XBF
CSB-EP003077XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Serine protease HTRA1 (htra1) CSB-YP010901XBF
CSB-EP010901XBF
CSB-BP010901XBF
CSB-MP010901XBF
CSB-EP010901XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Surfeit locus protein 1 (surf1), partial CSB-YP022963XBF1
CSB-EP022963XBF1
CSB-BP022963XBF1
CSB-MP022963XBF1
CSB-EP022963XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Rab-like protein 3 (rabl3) CSB-YP019239XBF
CSB-EP019239XBF
CSB-BP019239XBF
CSB-MP019239XBF
CSB-EP019239XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Cullin-3 (cul3), partial CSB-YP006220XBF
CSB-EP006220XBF
CSB-BP006220XBF
CSB-MP006220XBF
CSB-EP006220XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Mitochondrial ribonuclease P protein 1 (rg9mtd1) CSB-YP019619XBF
CSB-EP019619XBF
CSB-BP019619XBF
CSB-MP019619XBF
CSB-EP019619XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Dual specificity phosphatase DUPD1 (dupd1) CSB-YP007232XBF
CSB-EP007232XBF
CSB-BP007232XBF
CSB-MP007232XBF
CSB-EP007232XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Glucosamine-6-phosphate isomerase 2 (gnpda2) CSB-YP009631XBF
CSB-EP009631XBF
CSB-BP009631XBF
CSB-MP009631XBF
CSB-EP009631XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Low-density lipoprotein receptor class A domain-containing protein 3 (ldlrad3), partial CSB-YP012849XBF1
CSB-EP012849XBF1
CSB-BP012849XBF1
CSB-MP012849XBF1
CSB-EP012849XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Breast cancer metastasis-suppressor 1-like protein (brms1l) CSB-YP002815XBF
CSB-EP002815XBF
CSB-BP002815XBF
CSB-MP002815XBF
CSB-EP002815XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis CCR4-NOT transcription complex subunit 7 (cnot7) CSB-YP005672XBF
CSB-EP005672XBF
CSB-BP005672XBF
CSB-MP005672XBF
CSB-EP005672XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Zinc transporter ZIP3 (slc39a3), partial CSB-YP021632XBF
CSB-EP021632XBF
CSB-BP021632XBF
CSB-MP021632XBF
CSB-EP021632XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Phosphatidylinositol 4-kinase beta (pi4kb), partial CSB-YP017956XBF
CSB-EP017956XBF
CSB-BP017956XBF
CSB-MP017956XBF
CSB-EP017956XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Tetratricopeptide repeat protein 26 (ttc26), partial CSB-YP025219XBF
CSB-EP025219XBF
CSB-BP025219XBF
CSB-MP025219XBF
CSB-EP025219XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Target of rapamycin complex 2 subunit MAPKAP1 (mapkap1), partial CSB-YP013472XBF
CSB-EP013472XBF
CSB-BP013472XBF
CSB-MP013472XBF
CSB-EP013472XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Olfactomedin-like protein 2A (olfml2a), partial CSB-YP016325XBF
CSB-EP016325XBF
CSB-BP016325XBF
CSB-MP016325XBF
CSB-EP016325XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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