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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Ferrochelatase 2 (hemH2) CSB-YP748124BQN
CSB-EP748124BQN
CSB-BP748124BQN
CSB-MP748124BQN
CSB-EP748124BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Serine-protein kinase rsbW (rsbW) CSB-YP748125BQN
CSB-EP748125BQN
CSB-BP748125BQN
CSB-MP748125BQN
CSB-EP748125BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein H 1 (sspH1) CSB-YP748126BQN
CSB-EP748126BQN
CSB-BP748126BQN
CSB-MP748126BQN
CSB-EP748126BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Processive diacylglycerol glucosyltransferase (ugtP) CSB-YP748127BQN
CSB-EP748127BQN
CSB-BP748127BQN
CSB-MP748127BQN
CSB-EP748127BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA topoisomerase 3 (topB), partial CSB-YP748128BQN
CSB-EP748128BQN
CSB-BP748128BQN
CSB-MP748128BQN
CSB-EP748128BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP748129BQN
CSB-EP748129BQN
CSB-BP748129BQN
CSB-MP748129BQN
CSB-EP748129BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP748130BQN
CSB-EP748130BQN
CSB-BP748130BQN
CSB-MP748130BQN
CSB-EP748130BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DEAD-box ATP-dependent RNA helicase CshA (cshA), partial CSB-YP748131BQN
CSB-EP748131BQN
CSB-BP748131BQN
CSB-MP748131BQN
CSB-EP748131BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoglucosamine mutase (glmM) CSB-YP748132BQN
CSB-EP748132BQN
CSB-BP748132BQN
CSB-MP748132BQN
CSB-EP748132BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus tRNA pseudouridine synthase A 1 (truA1) CSB-YP748133BQN
CSB-EP748133BQN
CSB-BP748133BQN
CSB-MP748133BQN
CSB-EP748133BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L30 (rpmD) CSB-YP748134BQN
CSB-EP748134BQN
CSB-BP748134BQN
CSB-MP748134BQN
CSB-EP748134BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S19 (rpsS) CSB-YP748135BQN
CSB-EP748135BQN
CSB-BP748135BQN
CSB-MP748135BQN
CSB-EP748135BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Elongation factor G (fusA), partial CSB-YP748136BQN
CSB-EP748136BQN
CSB-BP748136BQN
CSB-MP748136BQN
CSB-EP748136BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glutamate--tRNA ligase (gltX) CSB-YP748137BQN
CSB-EP748137BQN
CSB-BP748137BQN
CSB-MP748137BQN
CSB-EP748137BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative ATP:guanido phosphotransferase BCE_0080 (BCE_0080) CSB-YP748138BQN
CSB-EP748138BQN
CSB-BP748138BQN
CSB-MP748138BQN
CSB-EP748138BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Peptidyl-tRNA hydrolase (pth) CSB-YP748139BQN
CSB-EP748139BQN
CSB-BP748139BQN
CSB-MP748139BQN
CSB-EP748139BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP748140BQN
CSB-EP748140BQN
CSB-BP748140BQN
CSB-MP748140BQN
CSB-EP748140BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP748141BQN
CSB-EP748141BQN
CSB-BP748141BQN
CSB-MP748141BQN
CSB-EP748141BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (Ile)-lysidine synthase (tilS) CSB-YP748142WAQ
CSB-EP748142WAQ
CSB-BP748142WAQ
CSB-MP748142WAQ
CSB-EP748142WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferrochelatase (hemH) CSB-YP748143WAQ
CSB-EP748143WAQ
CSB-BP748143WAQ
CSB-MP748143WAQ
CSB-EP748143WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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