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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Probable tRNA sulfurtransferase (thiI) CSB-YP748084BQN
CSB-EP748084BQN
CSB-BP748084BQN
CSB-MP748084BQN
CSB-EP748084BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Malate dehydrogenase (mdh) CSB-YP748085BQN
CSB-EP748085BQN
CSB-BP748085BQN
CSB-MP748085BQN
CSB-EP748085BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L20 (rplT) CSB-YP748086BQN
CSB-EP748086BQN
CSB-BP748086BQN
CSB-MP748086BQN
CSB-EP748086BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Cell division protein ZapA (zapA) CSB-YP748087BQN
CSB-EP748087BQN
CSB-BP748087BQN
CSB-MP748087BQN
CSB-EP748087BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP748088BQN
CSB-EP748088BQN
CSB-BP748088BQN
CSB-MP748088BQN
CSB-EP748088BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Queuine tRNA-ribosyltransferase (tgt) CSB-YP748089BQN
CSB-EP748089BQN
CSB-BP748089BQN
CSB-MP748089BQN
CSB-EP748089BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Elongation factor 4 (lepA), partial CSB-YP748090BQN
CSB-EP748090BQN
CSB-BP748090BQN
CSB-MP748090BQN
CSB-EP748090BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L33 2 (rpmG2) CSB-YP748091BQN
CSB-EP748091BQN
CSB-BP748091BQN
CSB-MP748091BQN
CSB-EP748091BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP748092BQN
CSB-EP748092BQN
CSB-BP748092BQN
CSB-MP748092BQN
CSB-EP748092BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP748093BQN
CSB-EP748093BQN
CSB-BP748093BQN
CSB-MP748093BQN
CSB-EP748093BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glucosamine-6-phosphate deaminase (nagB) CSB-YP748094BQN
CSB-EP748094BQN
CSB-BP748094BQN
CSB-MP748094BQN
CSB-EP748094BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0637 protein BCE_4007 (BCE_4007) CSB-YP748095BQN
CSB-EP748095BQN
CSB-BP748095BQN
CSB-MP748095BQN
CSB-EP748095BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L32 (rpmF) CSB-YP748096BQN
CSB-EP748096BQN
CSB-BP748096BQN
CSB-MP748096BQN
CSB-EP748096BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase 1 (murG1) CSB-YP748097BQN
CSB-EP748097BQN
CSB-BP748097BQN
CSB-MP748097BQN
CSB-EP748097BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Lipoprotein signal peptidase (lspA), partial CSB-YP748098BQN1
CSB-EP748098BQN1
CSB-BP748098BQN1
CSB-MP748098BQN1
CSB-EP748098BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP748099BQN
CSB-EP748099BQN
CSB-BP748099BQN
CSB-MP748099BQN
CSB-EP748099BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0122 protein BCE_3888 (BCE_3888) CSB-YP748100BQN
CSB-EP748100BQN
CSB-BP748100BQN
CSB-MP748100BQN
CSB-EP748100BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosome-recycling factor (frr) CSB-YP748101BQN
CSB-EP748101BQN
CSB-BP748101BQN
CSB-MP748101BQN
CSB-EP748101BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S15 (rpsO) CSB-YP748102BQN
CSB-EP748102BQN
CSB-BP748102BQN
CSB-MP748102BQN
CSB-EP748102BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Gag-Pol polyprotein (gag-pol), partial CSB-YP748103HDAK
CSB-EP748103HDAK
CSB-BP748103HDAK
CSB-MP748103HDAK
CSB-EP748103HDAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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