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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 30S ribosomal protein S12 (rpsL) CSB-YP748164WAQ
CSB-EP748164WAQ
CSB-BP748164WAQ
CSB-MP748164WAQ
CSB-EP748164WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola 50S ribosomal protein L33 (rpmG) CSB-YP748165TPP
CSB-EP748165TPP
CSB-BP748165TPP
CSB-MP748165TPP
CSB-EP748165TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola ATP-dependent Clp protease proteolytic subunit 2 (clpP2) CSB-YP748166TPP
CSB-EP748166TPP
CSB-BP748166TPP
CSB-MP748166TPP
CSB-EP748166TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP748167TPP
CSB-EP748167TPP
CSB-BP748167TPP
CSB-MP748167TPP
CSB-EP748167TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Flagellar assembly factor FliW (fliW) CSB-YP748168TPP
CSB-EP748168TPP
CSB-BP748168TPP
CSB-MP748168TPP
CSB-EP748168TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola UPF0102 protein TDE_2303 (TDE_2303) CSB-YP748169TPP
CSB-EP748169TPP
CSB-BP748169TPP
CSB-MP748169TPP
CSB-EP748169TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Galactose/methyl galactoside import ATP-binding protein MglA (mglA) CSB-YP748170TPP
CSB-EP748170TPP
CSB-BP748170TPP
CSB-MP748170TPP
CSB-EP748170TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Lipoprotein-releasing system ATP-binding protein LolD (lolD) CSB-YP748171TPP
CSB-EP748171TPP
CSB-BP748171TPP
CSB-MP748171TPP
CSB-EP748171TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP748172TPP
CSB-EP748172TPP
CSB-BP748172TPP
CSB-MP748172TPP
CSB-EP748172TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola GTPase obg (obg) CSB-YP748173TPP
CSB-EP748173TPP
CSB-BP748173TPP
CSB-MP748173TPP
CSB-EP748173TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola 30S ribosomal protein S18 (rpsR) CSB-YP748174TPP
CSB-EP748174TPP
CSB-BP748174TPP
CSB-MP748174TPP
CSB-EP748174TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Dephospho-CoA kinase (coaE) CSB-YP748175TPP
CSB-EP748175TPP
CSB-BP748175TPP
CSB-MP748175TPP
CSB-EP748175TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Ribonuclease HII (rnhB) CSB-YP748176TPP
CSB-EP748176TPP
CSB-BP748176TPP
CSB-MP748176TPP
CSB-EP748176TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Phosphopantetheine adenylyltransferase (coaD) CSB-YP748177TPP
CSB-EP748177TPP
CSB-BP748177TPP
CSB-MP748177TPP
CSB-EP748177TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP748178TPP
CSB-EP748178TPP
CSB-BP748178TPP
CSB-MP748178TPP
CSB-EP748178TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP748179TPP
CSB-EP748179TPP
CSB-BP748179TPP
CSB-MP748179TPP
CSB-EP748179TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Threonine--tRNA ligase (thrS), partial CSB-YP748180TPP
CSB-EP748180TPP
CSB-BP748180TPP
CSB-MP748180TPP
CSB-EP748180TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Ribonuclease 3 (rnc) CSB-YP748181TPP
CSB-EP748181TPP
CSB-BP748181TPP
CSB-MP748181TPP
CSB-EP748181TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola DNA polymerase IV (dinB) CSB-YP748182TPP
CSB-EP748182TPP
CSB-BP748182TPP
CSB-MP748182TPP
CSB-EP748182TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP748183TPP
CSB-EP748183TPP
CSB-BP748183TPP
CSB-MP748183TPP
CSB-EP748183TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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