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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chromobacterium violaceum Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP749124CKA
CSB-EP749124CKA
CSB-BP749124CKA
CSB-MP749124CKA
CSB-EP749124CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP749125CKA
CSB-EP749125CKA
CSB-BP749125CKA
CSB-MP749125CKA
CSB-EP749125CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum NAD-dependent protein deacetylase (cobB) CSB-YP749126CKA
CSB-EP749126CKA
CSB-BP749126CKA
CSB-MP749126CKA
CSB-EP749126CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Glucokinase (glk) CSB-YP749127CKA
CSB-EP749127CKA
CSB-BP749127CKA
CSB-MP749127CKA
CSB-EP749127CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP749128CKA
CSB-EP749128CKA
CSB-BP749128CKA
CSB-MP749128CKA
CSB-EP749128CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl pyrophosphate synthase (uppS) CSB-YP749129RND
CSB-EP749129RND
CSB-BP749129RND
CSB-MP749129RND
CSB-EP749129RND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable mannan synthase 5 (CSLA5), partial CSB-YP749130OFG1
CSB-EP749130OFG1
CSB-BP749130OFG1
CSB-MP749130OFG1
CSB-EP749130OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter G family member 39 (ABCG39), partial CSB-YP749131DOA
CSB-EP749131DOA
CSB-BP749131DOA
CSB-MP749131DOA
CSB-EP749131DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Diamine acetyltransferase 2 (SAT2) CSB-YP749132PI
CSB-EP749132PI
CSB-BP749132PI
CSB-MP749132PI
CSB-EP749132PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Alpha-actinin, sarcomeric (Actn), partial CSB-YP749133BZL
CSB-EP749133BZL
CSB-BP749133BZL
CSB-MP749133BZL
CSB-EP749133BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Eukaryotic translation initiation factor 3 subunit C (eIF3-S8), partial CSB-YP749134BZL
CSB-EP749134BZL
CSB-BP749134BZL
CSB-MP749134BZL
CSB-EP749134BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Argininosuccinate synthase (AGAP003015) CSB-YP749135BZL
CSB-EP749135BZL
CSB-BP749135BZL
CSB-MP749135BZL
CSB-EP749135BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Probable cytosolic iron-sulfur protein assembly protein Ciao1 (Ciao1) CSB-YP749136BZL
CSB-EP749136BZL
CSB-BP749136BZL
CSB-MP749136BZL
CSB-EP749136BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Probable actin-related protein 2/3 complex subunit 2 (Arc-p34) CSB-YP749137BZL
CSB-EP749137BZL
CSB-BP749137BZL
CSB-MP749137BZL
CSB-EP749137BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Nuclear cap-binding protein subunit 1 (Cbp80), partial CSB-YP749138BZL
CSB-EP749138BZL
CSB-BP749138BZL
CSB-MP749138BZL
CSB-EP749138BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Phagocyte signaling-impaired protein (psidin), partial CSB-YP749139BZL
CSB-EP749139BZL
CSB-BP749139BZL
CSB-MP749139BZL
CSB-EP749139BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Coiled-coil domain-containing protein 22 homolog (AGAP011801), partial CSB-YP749140BZL
CSB-EP749140BZL
CSB-BP749140BZL
CSB-MP749140BZL
CSB-EP749140BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae DNA replication licensing factor Mcm6 (Mcm6), partial CSB-YP749141BZL
CSB-EP749141BZL
CSB-BP749141BZL
CSB-MP749141BZL
CSB-EP749141BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae tRNA (guanine-N (7)-)-methyltransferase (AGAP004752) CSB-YP749142BZL
CSB-EP749142BZL
CSB-BP749142BZL
CSB-MP749142BZL
CSB-EP749142BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Eukaryotic translation initiation factor 3 subunit L (AGAP006130), partial CSB-YP749143BZL
CSB-EP749143BZL
CSB-BP749143BZL
CSB-MP749143BZL
CSB-EP749143BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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