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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacter sulfurreducens Uncharacterized RNA methyltransferase GSU1452 (GSU1452) CSB-YP748264GBK
CSB-EP748264GBK
CSB-BP748264GBK
CSB-MP748264GBK
CSB-EP748264GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Bifunctional protein pyrR (pyrR) CSB-YP748265GBK
CSB-EP748265GBK
CSB-BP748265GBK
CSB-MP748265GBK
CSB-EP748265GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens S-methyl-5'-thioadenosine phosphorylase (mtnP) CSB-YP748266GBK
CSB-EP748266GBK
CSB-BP748266GBK
CSB-MP748266GBK
CSB-EP748266GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Glycogen synthase 1 (glgA1), partial CSB-YP748267GBK
CSB-EP748267GBK
CSB-BP748267GBK
CSB-MP748267GBK
CSB-EP748267GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens DNA ligase (ligA), partial CSB-YP748268GBK
CSB-EP748268GBK
CSB-BP748268GBK
CSB-MP748268GBK
CSB-EP748268GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 30S ribosomal protein S18 (rpsR) CSB-YP748269GBK
CSB-EP748269GBK
CSB-BP748269GBK
CSB-MP748269GBK
CSB-EP748269GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP748270GBK
CSB-EP748270GBK
CSB-BP748270GBK
CSB-MP748270GBK
CSB-EP748270GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Probable endonuclease 4 (nfo) CSB-YP748271GBK
CSB-EP748271GBK
CSB-BP748271GBK
CSB-MP748271GBK
CSB-EP748271GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens UPF0758 protein GSU0386 (GSU0386) CSB-YP748272GBK
CSB-EP748272GBK
CSB-BP748272GBK
CSB-MP748272GBK
CSB-EP748272GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens NADH-quinone oxidoreductase subunit A 1 (nuoA1), partial CSB-YP748273GBK1
CSB-EP748273GBK1
CSB-BP748273GBK1
CSB-MP748273GBK1
CSB-EP748273GBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens UPF0502 protein GSU0233 (GSU0233) CSB-YP748274GBK
CSB-EP748274GBK
CSB-BP748274GBK
CSB-MP748274GBK
CSB-EP748274GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens ATP synthase epsilon chain (atpC) CSB-YP748275GBK
CSB-EP748275GBK
CSB-BP748275GBK
CSB-MP748275GBK
CSB-EP748275GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Heat-inducible transcription repressor HrcA (hrcA) CSB-YP748276GBK
CSB-EP748276GBK
CSB-BP748276GBK
CSB-MP748276GBK
CSB-EP748276GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Deoxyadenosine kinase (LJ_1824) CSB-YP748277LAW
CSB-EP748277LAW
CSB-BP748277LAW
CSB-MP748277LAW
CSB-EP748277LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii S-ribosylhomocysteine lyase (luxS) CSB-YP748278LAW
CSB-EP748278LAW
CSB-BP748278LAW
CSB-MP748278LAW
CSB-EP748278LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Putative ABC transporter ATP-binding protein LJ_1704 (LJ_1704), partial CSB-YP748279LAW
CSB-EP748279LAW
CSB-BP748279LAW
CSB-MP748279LAW
CSB-EP748279LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Transcriptional repressor NrdR (nrdR) CSB-YP748280LAW
CSB-EP748280LAW
CSB-BP748280LAW
CSB-MP748280LAW
CSB-EP748280LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Acylphosphatase (acyP) CSB-YP748281LAW
CSB-EP748281LAW
CSB-BP748281LAW
CSB-MP748281LAW
CSB-EP748281LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii 50S ribosomal protein L21 (rplU) CSB-YP748282LAW
CSB-EP748282LAW
CSB-BP748282LAW
CSB-MP748282LAW
CSB-EP748282LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP748283LAW
CSB-EP748283LAW
CSB-BP748283LAW
CSB-MP748283LAW
CSB-EP748283LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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